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<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial">Dear All,<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"> I have couple of problems in the analysis my proteins using analytical ultracentrifugation.
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial">First one is that I am trying to analyse the association state of my protein complex that is formed in between two proteins that associate to form crystals in
buffer solutions. These crystals are about a few microns in size. So it is possible to study the association state of such big size particles using analytical ultracentrifugation. Also I do not know how to calculate the v bar of bigger size particles of about
500 nm is it independent of size? <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial">Second problem that I was having was when I was analysing a lipoprotein (protein having a hydrophobic lipid tail attached to it) does any one have any idea how
to analyse such samples ? <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"><o:p> </o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial">Any opinion or suggestion regarding the above problems that I am facing would be of great help to me. <o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"><o:p> </o:p></span></font></p>
<p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">Thanks
<o:p></o:p></span></font></p>
<p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt"><o:p> </o:p></span></font></p>
<p class="MsoAutoSig"><st1:PersonName w:st="on"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Prasad Satyamurthy</span></font></st1:PersonName>
<o:p></o:p></p>
<p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">Genomics and Biotechnology
<o:p></o:p></span></font></p>
<p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">D4 Chikalthana Aurangabad<o:p></o:p></span></font></p>
<p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">Email: psatyamurthy@wockhardt.com
<o:p></o:p></span></font></p>
<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt"><o:p> </o:p></span></font></p>
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