<html><head><meta http-equiv="Content-Type" content="text/html charset=windows-1252"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space;">Dear Prasad,<div><span class="Apple-tab-span" style="white-space:pre"> </span>You can combine the data from multiple speeds using the Program SEDANAL as described in the paper:</div><div><br></div><div>Biophys Chem. 2004 Mar 1;108(1-3):273-9.<br>"Sedimentation velocity, multi-speed method for analyzing polydisperse solutions."<br>Stafford WF, Braswell EH.</div><div><br></div><div>SEDANAL can be downloaded from <a href="http://sedanal.org/">http://sedanal.org/</a></div><div><br></div><div>I would be happy to help you get started.</div><div><br></div><div>Walter<br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px;"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Arial; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Arial; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">_________________________</div><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Walter Stafford<br><a href="mailto:wstafford3@walterstafford.com">wstafford3@walterstafford.com</a></div><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><br></div></span></div></span></span><br class="Apple-interchange-newline">
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<br><div><div>On May 20, 2014, at 02:05, Prasad Satyamurthy <<a href="mailto:psatyamurthy@wockhardt.com">psatyamurthy@wockhardt.com</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
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<div class="Section1"><p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial">Dear All,<o:p></o:p></span></font></p><p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"> I am analysing a suspension of protein molecule. This suspension has crystals and soluble form of the same protein. To analyze this suspension and
get information about the crystal size and stoke’s radius of the soluble form of the protein I am running this sample in a sedimentation velocity experiment.
<o:p></o:p></span></font></p><p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"> In order to sediment the larger crystals I initially started with 6000 rpm later increased it to 55000 rpm. All the data was acquired using the interference
detection system and was performed using the aluminium centre pieces with sapphire windows. <o:p></o:p></span></font></p><p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"> So I would like to know if this is the right way of analysing such type of samples. Also which model or fitting program would be appropriate for
such analysis? <o:p></o:p></span></font></p><p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt;
font-family:Arial"> </span></font></p><p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">Thanks and Regards
<o:p></o:p></span></font></p><p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt"> </span></font></p><p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt">Prasad Satyamurthy
<o:p></o:p></span></font></p><p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt"> </span></font></p><p class="MsoAutoSig"><font size="3" face="Times New Roman"><span style="font-size:
12.0pt"> </span></font></p><p class="MsoAutoSig"><font size="2" face="Times New Roman"><span style="font-size:
11.0pt">Disclaimer :<o:p></o:p></span></font></p><p class="MsoAutoSig"><font size="2" face="Times New Roman"><span style="font-size:
11.0pt"> </span></font></p><p class="MsoAutoSig"><font size="2" face="Times New Roman"><span style="font-size:
11.0pt">All the work done with respect to this experiement are my personal experiemntal work done using the Analytical ultracentrifuge and bears no relation with the company.
<o:p></o:p></span></font></p>
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