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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;"><span style="color: rgb(34, 34, 34); font-family: arial; font-size: small; background-color: rgb(255, 255, 255);">Hi Prasad!</span>
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<div style="color: rgb(34, 34, 34); font-family: arial; font-size: small;"> Are you a new user? If not, apologies if these are obvious, and someone much more experienced than I can help you!</div>
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<div style="color: rgb(34, 34, 34); font-family: arial; font-size: small;">1) Unless you've loaded the cell perfectly (and who can!) you will have 2 meniscus artifacts, one for the sample and one for the reference. You might want to make sure you've set the
meniscus at the sample meniscus and not the reference (it should be the positive spike).</div>
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<div style="color: rgb(34, 34, 34); font-family: arial; font-size: small;">2) If you are trying to fit right next to the meniscus, I would expect some high residuals there. However if you're trying to fit ~0.2 cm away from the meniscus and you're still getting
residuals greater than 0.02 OD, then that is a problem!</div>
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<div style="color: rgb(34, 34, 34); font-family: arial; font-size: small;">3) The other main cause of high residuals is an incorrect frictional ratio. If you've already fit the frictional ratio, you might try fitting to a c(s,f/f0) distribution. If each species
in your sample has a different frictional ratio, or if the frictional ratio isn't well determined, that could be a reason for your high residuals.</div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Post Doctoral Researcher</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Macromolecule and Vaccine Stabilization Center</span></font></span></font></div>
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<div id="divRpF371609" style="direction: ltr;"><font face="Tahoma" size="2" color="#000000"><b>From:</b> rasmb-bounces@list.rasmb.org [rasmb-bounces@list.rasmb.org] on behalf of Prasad Satyamurthy [psatyamurthy@wockhardt.com]<br>
<b>Sent:</b> Monday, March 10, 2014 6:53 AM<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] suggestion for improving analysis in case of high rmsd values and higher residuals near meniscus<br>
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<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Dear All,</span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial"> After I acquired Interference Data for my sample in my XLI Analytical Ultracentrifuge and fit using the continuous Cs model in sedfit I am not able
to get good quality fit as indicated by a high rmsd values also the residuals close to the meniscus is high. Can anyone suggest me how can improve how to improve my analysis?</span></font></p>
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<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Thanks in Advance
</span></font></p>
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<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial"> </span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Prasad Satyamurthy
</span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Wockhardt Research Centre</span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Maharashtra India
</span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Phone: +91 9987050315 </span></font></p>
<p class="MsoNormal"><font size="2" face="Arial"><span style="font-size:10.0pt; font-family:Arial">Email: psatyamurthy@wockhardt.com</span></font></p>
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