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<div style="direction: ltr;font-family: Tahoma;color: #000000;font-size: 10pt;">Hello Members!
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<div>Thank you all for your help in the past! I have a new question!<br>
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<div>Given that the standard procedure for characterizing a self association with sedimentation equilibrium is to sediment three different concentrations at three different rotor speeds, my question is this: Is there a standard procedure for characterizing
a hetero association?</div>
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<div><span style="font-size: 10pt;">Should I sediment three samples at different total protein concentration while keeping the molar ratio of antibody to ligand constant? Should I sediment three different molar ratios of antibody to ligand?</span></div>
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<div><span style="font-size: 10pt;">Some further information: We have an antibody-ligand system, and the ligand is 20 kDa. The ligand binds in the Fc region, so it's not necessarily limited to a divalent interaction.</span></div>
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<div><span style="font-size: 10pt;">Thanks again for your help I really appreciate it!</span></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">- Ronald Toth</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Post Doctoral Researcher</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">Macromolecule and Vaccine Stabilization Center</span></font></span></font></div>
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<div style="margin:0px"><font face="Times New Roman,serif" size="3"><span style="font-size:12pt"><font face="Tahoma,sans-serif" size="2"><span style="font-size:10pt">University of Kansas</span></font></span></font></div>
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