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<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D">Hi John,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D">We use – as default – f/f0 factor for globular proteins, which is 1.2 (not 1.5). However, user can freely enter any value for this factor in “Web
diffusion coefficient calculator” (<a href="https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences">https://www.biacore.com/lifesciences/Application_Support/online_support/Diffusion_Coefficient_Calculator/index.html?section=lifesciences&realsection=lifesciences</a>)<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D">You need to be registered on the Biacore website to access it.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D">Best regards,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D">Natalia<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><b><i><span lang="EN-US" style="color:#1F497D">Natalia Markova (PhD)
<o:p></o:p></span></i></b></p>
<p class="MsoNormal"><i><span lang="EN-US" style="color:#1F497D">GE Healthcare<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span lang="EN-US" style="color:#1F497D">R&D Life Sciences<o:p></o:p></span></i></p>
<p class="MsoNormal" style="text-autospace:none"><i><span lang="EN-US" style="color:#1F497D">Rapsgatan 23<o:p></o:p></span></i></p>
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<p class="MsoNormal"><i><span style="color:#1F497D">Sweden<o:p></o:p></span></i></p>
<p class="MsoNormal"><i><span style="color:#1F497D"><o:p> </o:p></span></i></p>
<p class="MsoNormal"><span style="color:#1F497D">M +46 727 33 58 80<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D">E <a href="mailto:natalia.markova@ge.com">
natalia.markova@ge.com</a><o:p></o:p></span></p>
<p class="MsoNormal"><i><span style="color:#1F497D"><o:p> </o:p></span></i></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#3366FF"><a href="https://exchange1.ad.slu.se/owa/redir.aspx?C=61606803db87492ba25280057e5d208e&URL=http%3a%2f%2fwww.biacore.com%2f" target="_blank" title="http://www.biacore.com/"><span lang="SV" style="color:#3366FF">www.biacore.com</span></a></span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#3366FF"><br>
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</span><span lang="EN-US" style="color:#1F497D">Disclaimer<o:p></o:p></span></p>
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this communication in error, please notify us immediately by phone. </span><span style="color:#1F497D">Thank you!<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span lang="EN-US" style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> rasmb-bounces@list.rasmb.org [mailto:rasmb-bounces@list.rasmb.org]
<b>On Behalf Of </b>John Sumida<br>
<b>Sent:</b> den 14 januari 2014 00:20<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] SPR query about CFCA applications<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span lang="EN-US" style="color:black">Dear RASMB,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="color:black">This question is in regards to an SPR application. We are currently investigating the use “calibration free concentration analysis” (CFCA) to monitor the stability of macromolecular preps over time
in terms of their activity. Input parameters for this analysis are ff0, viscosity, MW and D and we are using AUC to better determine both. Current applications seem to assume ff0=1.5, which may not be correct for monoclonal preps based on my reading of the
literature.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="color:black"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="color:black">My query.<o:p></o:p></span></p>
<p class="MsoListParagraph" style="text-indent:-18.0pt;mso-list:l0 level1 lfo2"><![if !supportLists]><span lang="EN-US" style="color:black"><span style="mso-list:Ignore">1.<span style="font:7.0pt "Times New Roman"">
</span></span></span><![endif]><span lang="EN-US" style="color:black">Is it currently the common practice to measure these quantities and if so what is the preferred method to do so?<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt;font-family:"Courier New""><o:p> </o:p></span></p>
<div>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt">Thank you all and Happy New Year,<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt"><o:p> </o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt">John Sumida, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt"><a href="http://depts.washington.edu/cidb4bio/index.shtml"><span style="color:windowtext">Analytical Biopharmacy Core Facility</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt">University of Washington<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt"><a href="http://www.moles.washington.edu/"><span style="color:windowtext">Molecular Engineering & Sciences Institute, G22</span></a><o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt">3946 West Stevens Way NE<o:p></o:p></span></p>
<p class="MsoNormal"><span lang="EN-US" style="font-size:12.0pt">Seattle WA 98195-1653<o:p></o:p></span></p>
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<p class="MsoNormal" style="text-autospace:none"><span lang="EN-US" style="color:black"><o:p> </o:p></span></p>
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