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<div class="moz-cite-prefix">On 10/25/13 10:47, Frank Niesen wrote:<br>
</div>
<blockquote
cite="mid:CANyJdN5Z38g0zoh_mjzE-50jqdXvnLV=02kcpNj9umqp7b_fsg@mail.gmail.com"
type="cite">
<div dir="ltr">Hello,
<div><br>
</div>
<div>I was wondering if anybody has experience with changing the
temperature in an equilibrium run at given speed, to
investigate temperature-dependent strength in association? Is
it feasible/sane to record data at equilibrium at, e.g., 20C,
and then to change the temperature to, e.g., 40C, and to hope
to get data for equilibrium and record once more?</div>
<div><br>
</div>
<div>In my case, the association seems to weaken substantially
with temperature and I observe a weaker curvature at the
higher temperature. For a third step, going back to the
original temperature, I programmed the run, but made two
mistakes with it - firstly to not allow sufficient time for
the equilibrium and, secondly, to somehow got the "stop AUC
after last scan" checked :o(</div>
<div>As result, I am looking at a scan that doesn't resemble the
one at lower temperature, yet it is similar to scans I saw
during the equilibration between the two temperatures at step
2.</div>
<div><br>
</div>
<div>Has anybody tried something similar? Am I onto something
believable/real, or shouldn't waste any more precious sample
on a proper repeat (i.e. separate runs/samples for each
temperature)?</div>
<div><br>
</div>
<div>Thanks in advance!</div>
<div>Frank</div>
</div>
<br>
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</pre>
</blockquote>
I refer you to Dimerization of Alpha-Chymotrypsin. II. Ionic
Strength and Temperature Dependence. Biochemistry 10, no.9, pp
1617-1622 (1971). Kirk C Aune, Lowell C. Goldsmith and Serge N.
Timasheff.<br>
<br>
This, of course, was with a Model E AUC. In order to get stable
temperature control above 30C with no oil condensation, we employed
an aluminum flashing liner on the chamber wall.<br>
<br>
Cheers.<br>
<br>
<pre class="moz-signature" cols="72">--
Kirk C Aune, Ph.D.
16289 Cambridge Court
Granger, IN 46530
(574)277-8589
Linux User #1720
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