<html><head><meta http-equiv="Content-Type" content="text/html charset=utf-8"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">I think if you want to be sure to be able to run at higher temperatures with interference optics, you should upgrade to a turbomolecular pump. It solved our problem completely. It leaves your chamber and lenses completely clean.<br><div apple-content-edited="true">
<span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Times; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Times; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: -webkit-auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; font-size: medium; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><br>___________________________<br>Walter Stafford<br><a href="mailto:wstafford3@walterstafford.com">wstafford3@walterstafford.com</a><br><br>“Life is short, Break the Rules.<br>Forgive quickly, Kiss SLOWLY.<br>Love truly. Laugh uncontrollably<br>And never regret ANYTHING<br>That makes you smile.”<br><br>― Mark Twain<br><br></div></span></span>
</div>
<br><div><div>On Oct 25, 2013, at 4:30 PM, Kirk C Aune <<a href="mailto:kaune@sbcglobal.net">kaune@sbcglobal.net</a>> wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
<meta content="text/html; charset=ISO-8859-1" http-equiv="Content-Type">
<div bgcolor="#FFFFFF" text="#000000">
<div class="moz-cite-prefix">On 10/25/13 10:47, Frank Niesen wrote:<br>
</div>
<blockquote cite="mid:CANyJdN5Z38g0zoh_mjzE-50jqdXvnLV=02kcpNj9umqp7b_fsg@mail.gmail.com" type="cite">
<div dir="ltr">Hello,
<div><br>
</div>
<div>I was wondering if anybody has experience with changing the
temperature in an equilibrium run at given speed, to
investigate temperature-dependent strength in association? Is
it feasible/sane to record data at equilibrium at, e.g., 20C,
and then to change the temperature to, e.g., 40C, and to hope
to get data for equilibrium and record once more?</div>
<div><br>
</div>
<div>In my case, the association seems to weaken substantially
with temperature and I observe a weaker curvature at the
higher temperature. For a third step, going back to the
original temperature, I programmed the run, but made two
mistakes with it - firstly to not allow sufficient time for
the equilibrium and, secondly, to somehow got the "stop AUC
after last scan" checked :o(</div>
<div>As result, I am looking at a scan that doesn't resemble the
one at lower temperature, yet it is similar to scans I saw
during the equilibration between the two temperatures at step
2.</div>
<div><br>
</div>
<div>Has anybody tried something similar? Am I onto something
believable/real, or shouldn't waste any more precious sample
on a proper repeat (i.e. separate runs/samples for each
temperature)?</div>
<div><br>
</div>
<div>Thanks in advance!</div>
<div>Frank</div>
</div>
<br>
<fieldset class="mimeAttachmentHeader"></fieldset>
<br>
<pre wrap="">_______________________________________________
RASMB mailing list
<a class="moz-txt-link-abbreviated" href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a>
<a class="moz-txt-link-freetext" href="http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org">http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</a>
</pre>
</blockquote>
I refer you to Dimerization of Alpha-Chymotrypsin. II. Ionic
Strength and Temperature Dependence. Biochemistry 10, no.9, pp
1617-1622 (1971). Kirk C Aune, Lowell C. Goldsmith and Serge N.
Timasheff.<br>
<br>
This, of course, was with a Model E AUC. In order to get stable
temperature control above 30C with no oil condensation, we employed
an aluminum flashing liner on the chamber wall.<br>
<br>
Cheers.<br>
<br>
<pre class="moz-signature" cols="72">--
Kirk C Aune, Ph.D.
16289 Cambridge Court
Granger, IN 46530
(574)277-8589
Linux User #1720
</pre>
</div>
_______________________________________________<br>RASMB mailing list<br><a href="mailto:RASMB@list.rasmb.org">RASMB@list.rasmb.org</a><br>http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org<br></blockquote></div><br></body></html>