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<div>Frank, if you think the association is weaker at higher temp then the relaxation will be primarily by diffusion if you go low to high temp. This will cause a very slow equilibration time. I suggest doing high to low temp in your case. Our experience is
that over a broad range of temp (5 to 20 to 30C) the time to equil is about the same if the system doesn't change much at each temperature.</div>
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<span style="font-weight:bold">From: </span>Frank Niesen <<a href="mailto:dr.frank.niesen@gmail.com">dr.frank.niesen@gmail.com</a>><br>
<span style="font-weight:bold">Date: </span>Friday, October 25, 2013 9:47 AM<br>
<span style="font-weight:bold">To: </span>"<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>" <<a href="mailto:rasmb@rasmb.org">rasmb@rasmb.org</a>><br>
<span style="font-weight:bold">Subject: </span>[RASMB] varying temperature during an equilibrium run<br>
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<div dir="ltr">Hello,
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<div>I was wondering if anybody has experience with changing the temperature in an equilibrium run at given speed, to investigate temperature-dependent strength in association? Is it feasible/sane to record data at equilibrium at, e.g., 20C, and then to change
the temperature to, e.g., 40C, and to hope to get data for equilibrium and record once more?</div>
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<div>In my case, the association seems to weaken substantially with temperature and I observe a weaker curvature at the higher temperature. For a third step, going back to the original temperature, I programmed the run, but made two mistakes with it - firstly
to not allow sufficient time for the equilibrium and, secondly, to somehow got the "stop AUC after last scan" checked :o(</div>
<div>As result, I am looking at a scan that doesn't resemble the one at lower temperature, yet it is similar to scans I saw during the equilibration between the two temperatures at step 2.</div>
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<div>Has anybody tried something similar? Am I onto something believable/real, or shouldn't waste any more precious sample on a proper repeat (i.e. separate runs/samples for each temperature)?</div>
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<div>Thanks in advance!</div>
<div>Frank</div>
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