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<font class="Apple-style-span" face="Arial" size="3">Hi Frank,</font>
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<div><font class="Apple-style-span" face="Arial" size="3">We tried that some time ago and found that the key was to have lots of patience for attainment of equilibrium and of course, a very stable sample. Here's a description:</font></div>
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<div><font class="Apple-style-span" face="Arial" size="3"> M. A. Daugherty,<span style="font: 7.0px Helvetica"> </span>M. Brenowitz, <span style="font: 7.0px Helvetica"> </span>and M. G. Fried (2000) Participation of the Amino-Terminal Domain in the Self-Association
of the </font><span class="Apple-style-span" style="font-family: Arial; font-size: medium; ">Full-Length Yeast TATA Binding Protein. Biochemistry 39, 4869-4880. </span></div>
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<div><font class="Apple-style-span" face="Arial" size="3">Good luck!</font></div>
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<div><font class="Apple-style-span" face="Arial" size="3">Mike Fried</font></div>
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<div id="divRpF153013" style="direction: ltr; "><font face="Tahoma" size="2" color="#000000"><b>From:</b> rasmb-bounces@list.rasmb.org [rasmb-bounces@list.rasmb.org] on behalf of Frank Niesen [dr.frank.niesen@gmail.com]<br>
<b>Sent:</b> Friday, October 25, 2013 10:47 AM<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] varying temperature during an equilibrium run<br>
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<div dir="ltr">Hello,
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<div>I was wondering if anybody has experience with changing the temperature in an equilibrium run at given speed, to investigate temperature-dependent strength in association? Is it feasible/sane to record data at equilibrium at, e.g., 20C, and then to change
the temperature to, e.g., 40C, and to hope to get data for equilibrium and record once more?</div>
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<div>In my case, the association seems to weaken substantially with temperature and I observe a weaker curvature at the higher temperature. For a third step, going back to the original temperature, I programmed the run, but made two mistakes with it - firstly
to not allow sufficient time for the equilibrium and, secondly, to somehow got the "stop AUC after last scan" checked :o(</div>
<div>As result, I am looking at a scan that doesn't resemble the one at lower temperature, yet it is similar to scans I saw during the equilibration between the two temperatures at step 2.</div>
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<div>Has anybody tried something similar? Am I onto something believable/real, or shouldn't waste any more precious sample on a proper repeat (i.e. separate runs/samples for each temperature)?</div>
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<div>Thanks in advance!</div>
<div>Frank</div>
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