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<DIV dir=ltr align=left><SPAN class=818425118-09082013><FONT color=#0000ff
size=2 face=Arial>John, unfortunately I think David is correct that SEDNTERP 2
has some bugs. I didn't reproduce your results, but in my case after entering
the correct vbar, density and viscosity it is always returning identical values
for s* and s(20,w). That is, it seems to be paying no attention to the new
values I entered on the solvent and sample page and still using the default
water values. I suspect this is an order of entry
problem.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=818425118-09082013></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=818425118-09082013><FONT color=#0000ff
size=2 face=Arial>Using SEDNTERP</FONT> <FONT color=#0000ff size=2
face=Arial>1.09 and entering your values I get that s* = 16.39 corresponds to
s(20,w) = 17.397. FYI if you are running Windows 7 you can run SEDNTERP 1.09
under the Windows XP virtual machine.</FONT></SPAN></DIV>
<DIV><SPAN class=818425118-09082013><FONT color=#0000ff size=2
face=Arial></FONT></SPAN> </DIV>
<DIV><SPAN class=818425118-09082013><FONT color=#0000ff size=2
face=Arial>John</FONT></SPAN></DIV>
<DIV dir=ltr align=left><FONT color=#0000ff size=2
face=Arial></FONT> </DIV>
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<FONT size=2 face=Tahoma><B>From:</B> rasmb-bounces@list.rasmb.org
[mailto:rasmb-bounces@list.rasmb.org] <B>On Behalf Of </B>John
Sumida<BR><B>Sent:</B> Friday, August 09, 2013 10:44 AM<BR><B>To:</B>
drdavidbhayes@yahoo.com<BR><B>Cc:</B> rasmb@rasmb.org<BR><B>Subject:</B> Re:
[RASMB] s20w calculation<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV class=WordSection1>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">Hi David,<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">Thanks for taking the time to
respond. I’m attempting to respond to your post. Apologies if I’m committing
some type of etiquette violation in responding to the group, but as you
requested some clarification as to my process I thought to respond
publicly.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">To answer your
questions.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">I’ve been trying to understand
why this particular polymer material produces a large s20w value when another
material with different surface charge properties produces an s20w of 7.9s and
sediments very differently.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">So the back calculation, going
from s20w to s*, was me playing with the data to see if I could go from the 17.3
s20w back to s* which I would have hoped – as you point out – would not have
been much different from 17.3s.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">My initial calculation using
the s20w correction equation (equation 19) was comforting in that the
calculated s* was 16.4s. I used SEDFIT to perform the same calculation
just to check that all was in order – and as I stated SEDFIT returned the 16.4s
value as had the calculation and the analysis in SEDANAL. Note that vbar
and solvent parameters had been entered in into the experiment file and gOfs
files had been generated by running DCDT. So I believe the s values
returned in SEDNAL are s20w values.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">The problem arose when I used
the Experiment page in SEDNTERP (version 20120828 BETA). Using the calculated
value of s*, SEDNTERP did not return the same value for s20w that SEDANAL
output. <o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">I also attempted the same
operation using the web version of SEDNTERP which resulted in the same result,
namely calculating an s20w of 27.8s when the experimentally observed s20w
was17.3s.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">I had not thought to turn off
the s20w calculation (in order to do so do I just remove vbar from the
experiment info?) and attempt to fit for s*. Will try that
next.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">Best
regards,<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">John Sumida,
Ph.D.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><A
href="http://depts.washington.edu/cidb4bio/index.shtml"><SPAN
style="COLOR: windowtext">Analytical Biopharmacy Core
Facility</SPAN></A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">University of
Washington<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt"><A
href="http://www.moles.washington.edu/"><SPAN
style="COLOR: windowtext">Molecular Engineering & Sciences Institute,
G22</SPAN></A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">3946 West Stevens Way
NE<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-SIZE: 14pt">Seattle WA
98195-1653<o:p></o:p></SPAN></P></DIV></BODY></HTML>