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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal><span style='font-size:14.0pt'>Dear RASMB<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>Wondering about the s020w calculation in SEDNTERP <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>For one of our polymer micelles with which we’ve been working, a SEDANAL analysis returns an s20w value (not corrected for concentration) of 17.3.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>If I use equation 19 in cited in the SEDNTERP documentation, from the s20w value=17.3, I calculate s* = 16.4s. <o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>Phsyical parameters used: <o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='font-size:14.0pt'><span style='mso-list:Ignore'>1.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-size:14.0pt'>experiment temp=20oC, <o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='font-size:14.0pt'><span style='mso-list:Ignore'>2.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-size:14.0pt'>vbar=0.8312 (measured by densitometry at 20oC)<o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='font-size:14.0pt'><span style='mso-list:Ignore'>3.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-size:14.0pt'>density of solution 1.00506 gms/cm3 (measured)<o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='font-size:14.0pt'><span style='mso-list:Ignore'>4.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-size:14.0pt'>viscosity 0.010281 (measured)<o:p></o:p></span></p><p class=MsoListParagraph style='text-indent:-.25in;mso-list:l0 level1 lfo1'><![if !supportLists]><span style='font-size:14.0pt'><span style='mso-list:Ignore'>5.<span style='font:7.0pt "Times New Roman"'> </span></span></span><![endif]><span style='font-size:14.0pt'>Assuming viscosity of water to be 0.01002, and the density to be 0.998234<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>However if I use the Experiment page in the SEDNTERP and enter value of s* calculated above, then s20w returned is 27.8 which is much higher than the value returned from SEDANAL. In fact in order to get SEDNTERP to return a value of 17.3s, I have to decrease S* to be 2.91s.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>In SEDFIT, if I input 16.4s, calculated from Equation 19 in the SEDNTERP documentation, and input the same physical parameter listed above, I get 17.4s in agreement with the SEDANAL result.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>I think in both SEDNTERP and SEDFIT, the same equation is used to correct s* to s20w, so I’m not clear what I’m missing.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>Any suggestions and comments are welcome and thank you, in advance, for taking the time to consider my question.<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>Best regards,<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><o:p> </o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>John Sumida<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><a href="http://depts.washington.edu/cidb4bio/index.shtml"><span style='color:windowtext'>Analytical Biopharmacy Core Facility</span></a><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>University of Washington<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'><a href="http://www.moles.washington.edu/"><span style='color:windowtext'>Molecular Engineering & Sciences Institute, G22</span></a><o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>3946 West Stevens Way NE<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:14.0pt'>Seattle WA 98195-1653<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>