<html><head><base href="x-msg://118/"></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hello All in RASMB land,<div><br><div>We have both the viscostar and the Anton Paar viscometer in the NCMH, and have used both to try and attain viscosity increments. In practice, only the viscostar actually works at reasonable concentrations (c.a. 1 mg/ml) for proteins. We've used this to look at conformations of intrinsically disordered proteins:</div><div><br></div><div><span class="Apple-style-span" style="font-size: 24px; "><font class="Apple-style-span" face="Arial" size="3">Rajeskar, K.V., Muntaha, S.T., Tame, J.R.H., Wharton, C.M.,
Thomas, C.M., White, S.A., Hyde, E.I. and Scott, D.J. (2010).
Order and
disorder in the domain organisation of the plasmid partition protein KorB. </font></span><span class="Apple-style-span" style="font-family: Arial; font-size: medium; "><i style="font-weight: normal; ">J. Biol. Chem</i><span class="Apple-style-span" style="font-weight: normal;">. </span><span class="slug-vol"><span lang="EN"><b>285</b></span></span><span class="slug-vol" style="font-weight: normal; "><span lang="EN"> </span></span><span class="slug-pages3" style="font-weight: normal; "><span lang="EN">15440-15449</span></span></span></div><div><span class="Apple-style-span" style="font-family: Arial; font-size: medium; "><span class="slug-pages3" style="font-weight: normal; "><span lang="EN"><br></span></span></span></div><div>
<!--EndFragment--><div><div>It gives very nice results, especially coupled to SEC-MALLS. I would also add to Mattia's point that using absorbance to measure your protein concentration is so much more accurate than refractive index increments. </div><div><br></div><div>The Anton Paar system gives very good (3 d.p.) precision measurements of solution viscosities which are essential for accurate corrections of sedimentation velocity data, but it's use beyond this is limited, in my experience. I know others in the NCMH have tried to push it further with carbohydrate systems, but they tend to be more abundant in yields than proteins.</div><div><br></div><div>The only downside to the viscostar is a tendency to blockages, so needs to be used on a fairly constant basis, and washed through regularly. However, despite that caveat, I have been impressed with it (when it is working properly...).</div><div><br></div><div>Best</div><div><br></div><div>Dave Scott.</div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div><br></div><div>On 6 Jun 2013, at 17:22, Razinkov, Vladimir wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div lang="EN-US" link="blue" vlink="purple"><div class="WordSection1" style="page: WordSection1; "><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Dear all,<o:p></o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Another rather simple and high throughput method to measure viscosity of proteins at low volumes (~ 35 uL with 384-well plate) using DLS plate reader.<o:p></o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; line-height: 17.4pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; "><span style="font-size: 10pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed/19995543" title="Analytical biochemistry." style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Anal Biochem.</span></a></span><span style="font-size: 10pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 10pt; font-family: Arial, sans-serif; color: black; ">2010 Apr 1;399(1):141-3. doi: 10.1016/j.ab.2009.12.003. Epub 2009 Dec 6.<o:p></o:p></span></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 4.5pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; background-position: initial initial; background-repeat: initial initial; "><b><span style="font-size: 15pt; font-family: Arial, sans-serif; color: black; ">High-throughput dynamic light scattering method for measuring viscosity of concentrated protein solutions.<o:p></o:p></span></b></p><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; "><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=He%20F%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">He F</span></a>,</span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Becker%20GW%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Becker GW</span></a>,</span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Litowski%20JR%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Litowski JR</span></a>,</span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Narhi%20LO%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Narhi LO</span></a>,</span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Brems%20DN%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Brems DN</span></a>,</span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "> </span><span style="font-size: 11pt; font-family: Arial, sans-serif; color: black; "><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Razinkov%20VI%5BAuthor%5D&cauthor=true&cauthor_uid=19995543" style="color: blue; text-decoration: underline; "><span style="color: rgb(102, 0, 102); ">Razinkov VI</span></a>.<o:p></o:p></span></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 7.7pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; background-position: initial initial; background-repeat: initial initial; "><b><span style="font-size: 11pt; font-family: Arial, sans-serif; color: rgb(114, 65, 40); ">Source<o:p></o:p></span></b></p><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 6pt; line-height: 13.1pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; background-position: initial initial; background-repeat: initial initial; "><span style="font-size: 10pt; font-family: Arial, sans-serif; color: black; ">Formulation and Analytical Resources, Amgen Inc., Seattle, WA 98119, USA.<o:p></o:p></span></p><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; "><b><span style="font-size: 11pt; font-family: Arial, sans-serif; color: rgb(152, 87, 53); ">Abstract<o:p></o:p></span></b></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 6pt; line-height: 12.75pt; background-image: initial; background-attachment: initial; background-origin: initial; background-clip: initial; background-color: white; background-position: initial initial; background-repeat: initial initial; "><span style="font-size: 10pt; font-family: Arial, sans-serif; color: black; ">We propose a new method to measure the viscosity of concentrated protein solutions in a high-throughput format. This method measures the apparent hydrodynamic radius of polystyrene beads with known sizes using a dynamic light scattering (DLS) system with a microplate reader. Glycerol solution viscosities obtained by the DLS method were in good agreement with those reported in the literature. Viscosity of the solutions of two monoclonal antibody molecules was acquired using both DLS and cone-and-plate techniques, and the results were comparable. The DLS method described here has the potential to be used in many aspects of protein characterization<o:p></o:p></span></p><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Regards<o:p></o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); ">Vladimir Razinkov<o:p></o:p></span></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 11pt; font-family: Calibri, sans-serif; color: rgb(31, 73, 125); "><o:p> </o:p></span></div><div><div style="border-right-style: none; border-bottom-style: none; border-left-style: none; border-width: initial; border-color: initial; border-top-style: solid; border-top-color: rgb(181, 196, 223); border-top-width: 1pt; padding-top: 3pt; padding-right: 0in; padding-bottom: 0in; padding-left: 0in; "><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; ">From:</span></b><span style="font-size: 10pt; font-family: Tahoma, sans-serif; "><span class="Apple-converted-space"> </span><a href="mailto:rasmb-bounces@list.rasmb.org" style="color: blue; text-decoration: underline; ">rasmb-bounces@list.rasmb.org</a><span class="Apple-converted-space"> </span>[mailto:rasmb-bounces@list.rasmb.org]<span class="Apple-converted-space"> </span><b>On Behalf Of<span class="Apple-converted-space"> </span></b>Richard Kingston<br><b>Sent:</b><span class="Apple-converted-space"> </span>Wednesday, June 05, 2013 9:15 PM<br><b>To:</b><span class="Apple-converted-space"> </span><a href="mailto:rasmb@rasmb.org" style="color: blue; text-decoration: underline; ">rasmb@rasmb.org</a><br><b>Subject:</b><span class="Apple-converted-space"> </span>[RASMB] Viscometer<o:p></o:p></span></div></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; ">Dear all,<o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; ">I've become interested in measuring the viscosity of protein solutions at varying temperature, and wondered if anyone on the list could advise on the best way to do this using commercially available instrumentation. While there are many ways to measure viscosity, for protein applications minimizing the required sample volume is pretty critical. I note that Anton Paar sell an instrument that measures both dynamic viscosity and density (SVM 3000 Stabinger viscometer). If you skip the density measurement, the sample requirements look almost "manageable". Cambridge also sell a micro-sample viscometer (Viscolab 5000) which uses microlitre volumes. <o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; ">I'm having difficulty tracking down people that might have used these or similar instruments, and could comment on the practicalities for protein work. If anyone can offer advice, either on- or off-list, it would be appreciated.<o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; ">Many thanks,<o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; ">Richard<o:p></o:p></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "> <o:p></o:p></div></div><div><div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">Richard Kingston, PhD.<o:p></o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">School of Biological Sciences<o:p></o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">The University of Auckland<o:p></o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">New Zealand<o:p></o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; ">website: <a href="http://persephone.sbs.auckland.ac.nz/richard/lab/" style="color: blue; text-decoration: underline; ">http://persephone.sbs.auckland.ac.nz/richard/lab/</a><o:p></o:p></span></div></div><div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></div></div><p class="MsoNormal" style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 12pt; "><span style="font-size: 9pt; font-family: Helvetica, sans-serif; color: black; "><o:p> </o:p></span></p></div></div><div style="margin-right: 0in; margin-left: 0in; font-size: 12pt; font-family: 'Times New Roman', serif; margin-top: 0in; margin-bottom: 0.0001pt; "><o:p> </o:p></div></div>_______________________________________________<br>RASMB mailing list<br><a href="mailto:RASMB@list.rasmb.org" style="color: blue; text-decoration: underline; ">RASMB@list.rasmb.org</a><br><a href="http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org" style="color: blue; text-decoration: underline; ">http://list.rasmb.org/listinfo.cgi/rasmb-rasmb.org</a><br></div></blockquote></div><br></div></div>
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