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<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Dear all,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Another rather simple and high throughput method to measure viscosity of proteins at low volumes (~ 35 uL with 384-well plate) using DLS plate reader.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal" style="line-height:17.4pt;background:white"><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed/19995543" title="Analytical biochemistry."><span style="color:#660066">Anal
Biochem.</span></a></span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">2010 Apr 1;399(1):141-3. doi: 10.1016/j.ab.2009.12.003. Epub 2009 Dec
6.<o:p></o:p></span></p>
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<b><span style="font-size:15.0pt;font-family:"Arial","sans-serif";color:black">High-throughput dynamic light scattering method for measuring viscosity of concentrated protein solutions.<o:p></o:p></span></b></p>
<p class="MsoNormal" style="background:white"><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=He%20F%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">He F</span></a>,</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Becker%20GW%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">Becker
GW</span></a>,</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Litowski%20JR%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">Litowski
JR</span></a>,</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Narhi%20LO%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">Narhi
LO</span></a>,</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Brems%20DN%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">Brems
DN</span></a>,</span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"> </span><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:black"><a href="http://www.ncbi.nlm.nih.gov/pubmed?term=Razinkov%20VI%5BAuthor%5D&cauthor=true&cauthor_uid=19995543"><span style="color:#660066">Razinkov
VI</span></a>.<o:p></o:p></span></p>
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<b><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#724128">Source<o:p></o:p></span></b></p>
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<span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">Formulation and Analytical Resources, Amgen Inc., Seattle, WA 98119, USA.<o:p></o:p></span></p>
<p class="MsoNormal" style="background:white"><b><span style="font-size:11.0pt;font-family:"Arial","sans-serif";color:#985735">Abstract<o:p></o:p></span></b></p>
<p class="MsoNormal" style="margin-bottom:6.0pt;line-height:12.75pt;background:white">
<span style="font-size:10.0pt;font-family:"Arial","sans-serif";color:black">We propose a new method to measure the viscosity of concentrated protein solutions in a high-throughput format. This method measures the apparent hydrodynamic radius of polystyrene
beads with known sizes using a dynamic light scattering (DLS) system with a microplate reader. Glycerol solution viscosities obtained by the DLS method were in good agreement with those reported in the literature. Viscosity of the solutions of two monoclonal
antibody molecules was acquired using both DLS and cone-and-plate techniques, and the results were comparable. The DLS method described here has the potential to be used in many aspects of protein characterization<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Regards
<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D">Vladimir Razinkov<o:p></o:p></span></p>
<p class="MsoNormal"><span style="font-size:11.0pt;font-family:"Calibri","sans-serif";color:#1F497D"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif"">From:</span></b><span style="font-size:10.0pt;font-family:"Tahoma","sans-serif""> rasmb-bounces@list.rasmb.org [mailto:rasmb-bounces@list.rasmb.org]
<b>On Behalf Of </b>Richard Kingston<br>
<b>Sent:</b> Wednesday, June 05, 2013 9:15 PM<br>
<b>To:</b> rasmb@rasmb.org<br>
<b>Subject:</b> [RASMB] Viscometer<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Dear all,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I've become interested in measuring the viscosity of protein solutions at varying temperature, and wondered if anyone on the list could advise on the best way to do this using commercially available instrumentation. While there are many
ways to measure viscosity, for protein applications minimizing the required sample volume is pretty critical. I note that Anton Paar sell an instrument that measures both dynamic viscosity and density (SVM 3000 Stabinger viscometer). If you skip the density
measurement, the sample requirements look almost "manageable". Cambridge also sell a micro-sample viscometer (Viscolab 5000) which uses microlitre volumes. <o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">I'm having difficulty tracking down people that might have used these or similar instruments, and could comment on the practicalities for protein work. If anyone can offer advice, either on- or off-list, it would be appreciated.<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Many thanks,<o:p></o:p></p>
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<p class="MsoNormal"><o:p> </o:p></p>
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<p class="MsoNormal">Richard<o:p></o:p></p>
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<p class="MsoNormal"> <o:p></o:p></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">Richard Kingston, PhD.<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">School of Biological Sciences<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">The University of Auckland<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">New Zealand<o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black">website: <a href="http://persephone.sbs.auckland.ac.nz/richard/lab/">http://persephone.sbs.auckland.ac.nz/richard/lab/</a><o:p></o:p></span></p>
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<p class="MsoNormal"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><span style="font-size:9.0pt;font-family:"Helvetica","sans-serif";color:black"><o:p> </o:p></span></p>
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