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<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">Dear RASMB,</span></font></p>
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<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">This is not directly related to AUC. I still believe that someone might be able to help.</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue"> </span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">We vary the amount of Ca in our protein solutions by having a constant amount of EGTA and add excess Ca to arrive at the level of Ca
we require for our biochemical assays. To calculate the free Ca in this EGTA-buffered system we use Maxchealater (<a href="http://www.stanford.edu/~cpatton/webmaxc/webmaxclite115.htm">http://www.stanford.edu/~cpatton/webmaxc/webmaxclite115.htm</a>).
</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue"> </span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">With some extra bit of money made available to us, we are planning to buy a Ca-meter along with a Ca-electrode and a reference electrode.
This will enable us to rule out the uncertainty in the Ca-levels arrived at by using the Maxchealater. When I explored I found Nova analytics (no commercial interest) have a Ca-meter and accessories. Unfortunately, I am not getting good pre-sales technical
update from the local vendor.</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue"> </span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">I want to measure Ca-levels as low as 100 to 10 nanoMolar. 1 mM will be the upper end. Could someone help?</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue"> </span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">Regards,</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue">Bala</span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Arial"><span style="font-size:12.0pt; font-family:Arial; color:blue"> </span></font></p>
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<p class="MsoNormal"><font size="2" color="blue" face="Arial"><span style="font-size:10.0pt; font-family:Arial; color:blue">##################################################</span></font><font color="blue"><span style="color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">Balakrishnan Kannan , Ph.D., </span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">Institute of Molecular and Cell Biology,</span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">BR Lab, Proteos, </span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">61, Biopolis Drive,</span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">Singapore</span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue">
138 673</span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span lang="FR" style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">e-mail:
</span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"><a href="mailto:bala@imcb.a-star.edu.sg" title="mailto:bala@imcb.a-star.edu.sg"><span lang="FR">bala@imcb.a-star.edu.sg</span></a></span></font><font color="blue" face="Comic Sans MS"><span lang="FR" style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">Ph: +65-6586 9831 </span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="3" color="blue" face="Comic Sans MS"><span style="font-size:12.0pt; font-family:"Comic Sans MS"; color:blue">Fax: +65-6779 1117 </span></font><font color="blue" face="Comic Sans MS"><span style="font-family:"Comic Sans MS"; color:blue"></span></font></p>
<p class="MsoNormal"><font size="2" color="blue" face="Arial"><span style="font-size:10.0pt; font-family:Arial; color:blue">##################################################</span></font></p>
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<p class="MsoNormal"><b><font size="2" face="Tahoma"><span style="font-size:10.0pt; font-family:Tahoma; font-weight:bold">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size:10.0pt; font-family:Tahoma"> rasmb-bounces@list.rasmb.org [mailto:rasmb-bounces@list.rasmb.org]
<b><span style="font-weight:bold">On Behalf Of </span></b>Jake Marold<br>
<b><span style="font-weight:bold">Sent:</span></b> Thursday, February 14, 2013 3:54 AM<br>
<b><span style="font-weight:bold">To:</span></b> John Burgner<br>
<b><span style="font-weight:bold">Cc:</span></b> rasmb@list.rasmb.org<br>
<b><span style="font-weight:bold">Subject:</span></b> Re: [RASMB] ProteomeLab startup errors</span></font></p>
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<p class="MsoNormal" style="margin-bottom:12.0pt"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">RASMB,<br>
<br>
Thank you to everyone who responded to my post for your comments and suggestions. I wanted to reply with an update to the problem we encountered yesterday, as we seem to have resolved the issue. As indicated in the README file associated with Version 6, problems
with the interference camera upon startup sometimes arise from problems with the frame grabber driver. I did not want to remove and uninstall the driver software files myself (mainly because I could not find back-ups to reinstall them in my immediate area).
Instead, I reset the camera configuration file MyCE2KE1.cam by running the SysReg utility. What I discovered was that the camera configuration file path was leading ProteomeLab to a directory, which did not include MyCE2KE1.cam. I simply corrected the path,
and was able to collect interference data overnight. We may never know why the path was spuriously changed, but at least there will now be a ?fix? in the RASMB thread for those encountering issues similar to these.<br>
<br>
There is an added bonus in that my run terminated due to a comport error. Fortunately it was towards the end of my data collection, but I don't think Version 6 is out of the water yet!<br>
<br>
-Jake Marold<br>
Johns Hopkins University</span></font></p>
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<p class="MsoNormal"><font size="3" face="Times New Roman"><span style="font-size:12.0pt">On Tue, Feb 12, 2013 at 8:27 PM, John Burgner <<a href="mailto:jburgner@purdue.edu" target="_blank">jburgner@purdue.edu</a>> wrote:</span></font></p>
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<p class="MsoNormal" style=""><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt; font-family:Calibri; color:#1F497D">I might in desperation remove the camera controller board (after unplugging the power for both) and clean the contacts
with a number 2 pencil and reseat all of the connectors. You have an advantage over the rest of us in that Erby Perdue lives in Baltimore and he probably will come see you before he comes to Richmond.</span></font></p>
<p class="MsoNormal" style=""><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt; font-family:Calibri; color:#1F497D">John Burgner</span></font></p>
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<p class="MsoNormal" style=""><font size="2" color="#1f497d" face="Calibri"><span style="font-size:11.0pt; font-family:Calibri; color:#1F497D"> </span></font></p>
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<p class="MsoNormal" style=""><b><font size="2" face="Tahoma"><span style="font-size:10.0pt; font-family:Tahoma; font-weight:bold">From:</span></font></b><font size="2" face="Tahoma"><span style="font-size:10.0pt; font-family:Tahoma">
<a href="mailto:rasmb-bounces@list.rasmb.org" target="_blank">rasmb-bounces@list.rasmb.org</a> [mailto:<a href="mailto:rasmb-bounces@list.rasmb.org" target="_blank">rasmb-bounces@list.rasmb.org</a>]
<b><span style="font-weight:bold">On Behalf Of </span></b>Jake Marold<br>
<b><span style="font-weight:bold">Sent:</span></b> Tuesday, February 12, 2013 5:39 PM<br>
<b><span style="font-weight:bold">To:</span></b> <a href="mailto:rasmb@list.rasmb.org" target="_blank">
rasmb@list.rasmb.org</a><br>
<b><span style="font-weight:bold">Subject:</span></b> [RASMB] ProteomeLab startup errors</span></font></p>
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<p class="MsoNormal" style=""><font size="3" face="Times New Roman"><span style="font-size:12.0pt">Hey everyone,<br>
<br>
This afternoon, we received two errors whilst attempting to startup our ProteomeLab software (we have version 6):<br>
<br>
Internal SDK error<br>
Source file: BFBrdUsr.c<br>
Line #: 1131<br>
Error Code 4298<br>
Message: Can't initialize for camera<br>
<br>
R2CamUsr.c<br>
Line #: 275<br>
Error Code 4298<br>
Message: Error reading configuration file<br>
<br>
When I attempt to open an old scan file, or create a new one, the interference box cannot be toggled in the cell setup, nor can I play with the laser settings. The comport connections are connected securely from the back of the ultracentrifuge, and the blue
lightning bolts flash, indicating connectivity. The only abnormality is that our battery backup surge protector (connected only to the computer, APC Back-UPS RS 900 model) has its replace battery light blinking and beeping. Given the error messages above,
I don't anticipate this is our problem, but I also do not know. <br>
<br>
Have any of you had any experience with an issue like this? I was hoping to perhaps receive a quick-fix tip from you before calling Beckman. Thanks in advance.</span></font></p>
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-Jake Marold<br>
Johns Hopkins University</span></font> </p>
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