<html><head></head><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><font class="Apple-style-span" color="#950f0b">Dear Carlos</font><div><font class="Apple-style-span" color="#950f0b"><br></font></div><div><font class="Apple-style-span" color="#950f0b">Can I just add one note to the clear expositions on this issue already contributed? Peter Budd's article on sedimentation of polyelectrolytes (in "Analytical Ultracentrifugation in Biochemistry
& Polymer Science" eds. S E Harding, J C Horton & A J Rowe), The
Royal Society of Chemistry, London) makes it depressingly clear that in pure water - as compared to very dilute salt solution - there is no simple extrapolation to infinite dilution available. </font></div><div><font class="Apple-style-span" color="#950f0b"><br></font></div><div><font class="Apple-style-span" color="#950f0b">Best of luck with your system!</font></div><div><font class="Apple-style-span" color="#950f0b"><br></font></div><div><font class="Apple-style-span" color="#950f0b">Arthur</font></div><div><br></div><div><br><div><div>On 23 Jul 2012, at 08:47, Carlos Alfonso Botello wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite">
<div>
Thank you very much for all your helpful answers. <br><br>
<br>
Carlos<br><br>
<br><br>
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Dear colleagues,<br><br>
We are running a 10 kDa protein at concentrations 0.2-2 mg/ml <b>in
water</b> (no buffer is present).The sedimentation coefficients that we
are obtaining are lower than expected for the monomer (1.4 - 1.5S). Is
there any correction of the S that we could apply to obtain the correct
S20,W.<br><br>
Thank you in advance for your help,<br><br>
Carlos<br><br>
<br>
<x-sigsep><p>
Dr. Carlos Alfonso<br>
Departamento Biologia Celular y Molecular<br>
Laboratorio de Interacciones Macromoleculares: Bioquímica
Citomimética<br>
Centro de Investigaciones Biológicas, CSIC<br>
C/ Ramiro de Maeztu, 9. <br>
28040-MADRID<br>
Tlfno.- 91 8373112 ext.4406-4408-4297 <br>
Fax: 915360432.<br>
E-mail: <a href="mailto:carlosa@cib.csic.es">carlosa@cib.csic.es</a><br>
<b>NEW lab web page: <br>
<a href="http://www.cib.csic.es/es/grupo.php?idgrupo=49" eudora="autourl">
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</a><font face="Webdings" size="6" color="#008000">P</font></b>
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</font><font face="Tahoma" size="1" color="#008000">Antes de imprimir este
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<div>Professor Arthur J Rowe<br>NCMH/Food Sciences<br>University of Nottingham<br>Sutton Bonington<br>Leics LE12 5RD UK<br><br>Tel: +44 115 9516156<br><a href="mailto:arthur.rowe@nottingham.ac.uk">arthur.rowe@nottingham.ac.uk</a><br></div><div><br></div><br class="Apple-interchange-newline">
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