<!DOCTYPE HTML PUBLIC "-//W3C//DTD HTML 4.0 Transitional//EN">
<HTML><HEAD>
<META content="text/html; charset=utf-8" http-equiv=Content-Type>
<META name=GENERATOR content="MSHTML 8.00.6001.19190">
<STYLE></STYLE>
</HEAD>
<BODY bgColor=#ffffff>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial>John,</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2><FONT face=Arial>The short answer is that you operate the AUC
<STRONG><U>very very carefully! </U><SPAN
style="FONT-FAMILY: 'Times New Roman'; FONT-SIZE: 12pt; mso-bidi-font-size: 10.0pt; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-US; mso-fareast-language: EN-US; mso-bidi-language: AR-SA"><FONT
color=#000000 size=4>☺</FONT></SPAN></STRONG></FONT></FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial><U></U></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial>But seriously, to my knowledge the only lab that puts BSL2
material into their XL-I had a special fume hood built that encloses the entire
instrument to deal with issues of sucking sample into the vacuum pump and
out into the room.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial>I have no knowledge of whether it is possible to filter the
output of the turbomolecular pump. But even if you solve that, another
non-trivial issue can sometimes be how to clean up and detoxify the centrifuge
cells? Remember that the cell housings inevitably get exposed to sample (at the
fill holes), and they <U>will not stand up to bleach</U>, which is the only
approved approach for detoxifying some biohazards.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial>Clearly the risk and safety issues depend on the particulars
of the sample, but if the sample in question is highly toxic or
infectious IMHO you should not put yourself, your co-workers, or the
operations of your core facility at risk.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><SPAN
class=828571801-29022012><FONT color=#0000ff size=2 face=Arial>FYI at APL our
approach to BSL2 is <U>just say no!</U><STRONG>
</STRONG></FONT></SPAN></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=828571801-29022012><FONT color=#0000ff
size=2 face=Arial>John</FONT></SPAN></DIV>
<DIV dir=ltr align=left>
<HR tabIndex=-1>
</DIV>
<DIV dir=ltr align=left><FONT size=2 face=Tahoma><B>From:</B>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf
Of </B>jpsumida<BR><B>Sent:</B> Tuesday, February 28, 2012 4:28 PM<BR><B>To:</B>
rasmb@rasmb.bbri.org<BR><B>Subject:</B> [RASMB] Biohazard Level
2<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV><FONT size=2 face=Arial>Dear RASMB,</FONT></DIV>
<DIV><FONT size=2 face=Arial></FONT> </DIV>
<DIV><FONT size=2 face=Arial>would like to ask about how one operates an AUC in
a Biohazard Level 2 environment. We have a Proteome XLI equipped
with a turbomolecular pump. According to the manufacturer, installing
a hepa filtration device in-line with the exhaust manifold could
generate a sufficiently high back-pressure that could very well
destroy the pump. According to the manufacturer, this has not been
done previously with an XLI equipped with a turbomolecular
pump.</FONT></DIV>
<DIV> </DIV>
<DIV><FONT size=2 face=Arial>Any advice and pointers are appreciated and my
thanks to all for your time in advance.</FONT></DIV>
<DIV> </DIV>
<DIV><FONT size=2 face=Arial>John Sumida<BR>Core Manager<BR>University of
Washington<BR></FONT></DIV></BODY></HTML>