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Dear Anna,<br>
<br>
maybe you have a bit of oil dust condensed on the lens of the
interference optics in the chamber. You can clean it with ethanol
and a tissue. Eventually you have to repeat this two or three times.
I check if its clean with the interference optics at low speed
through an empty rotor hole. We sometimes have this problem if the
measuring temperature differs from the room temperature. Most of the
time heating is worse.<br>
<br>
Hope this helps!<br>
Best regards<br>
Antje<br>
<br>
<br>
<br>
Am 26/01/2012 13:33, schrieb anna vitlin:
<blockquote
cite="mid:CAC69jLZG7k3+8+k_pCB+nEg5ovB5gcp4N-V0eH3b1h5xqCvAJQ@mail.gmail.com"
type="cite">
<div dir="ltr">
<div>Hello,</div>
<div> </div>
<div>We have a Beckmann XL-I running a An-60 Ti rotor. </div>
<div>When running sedimentation velocity experiments at 4 deg.
we have noticed protein-independent, time-independent,
radial-dependent noise, consistently between 6.2 and 6.4
Radius. </div>
<div>This phenomenon occurs using different cell assemblies,
different wavelengths and different velocities but does not
occur at 20 deg. </div>
<div>Has anyone detected behavior like this and how do we
troubleshoot it?</div>
<div>Should we call for service?</div>
<div> </div>
<div>Thank you</div>
<div>Anna Vitlin </div>
<div>Tel-Aviv university</div>
</div>
<br>
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</blockquote>
<br>
<pre class="moz-signature" cols="72">--
Antje Voelkel
Max Planck Institute of Colloids and Interfaces
Am Muehlenberg 1
D-14476 Potsdam
+49(0)331 567 9528/9531</pre>
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