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<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial>Peter, when you try to do a radial
calibration you have to be careful about having too high an OD in the
hole opposite the counterbalance (hole 2 in your case), and having a 6-channel
cell there rather than a 2-channel can also cause problems. This situation
arises because once you clear the radial calibration the instrument must try to
do the delay calibration using hole 2, since it no longer knows how to find
the very restricted holes (restricted radially) in the counterbalance.
It really expects to find a 2-channel cell in hole 2, and it tries to do the
delay calibration at 6.5 cm. The problem here might be that the OD of your
solutions in Channel B of cell 2 is too high so it can't find the center
rib, or the 'default' radial calibration is so far off that when it thinks it is
at 6.5 cm the light is actually hitting the Epon resin in between channels A and
B or between B and C.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial>This will always be true unless you have done a delay
calibration <U>before</U> you ask for the radial calibration. That is, you can
avoid this problem by first doing some sort of scan (setting the delay), and
<U>then</U> asking for a radial calibration.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial>Also be sure you haven't accidentally left the 400 nm cutoff
filter in place or at an in-between position.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial>You could temporarily put an empty 2-channel cell in
hole 2 and use that to do the radial calibration, then go back to your 6-channel
cell.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=175554820-01092011><FONT color=#0000ff
size=2 face=Arial>John</FONT></SPAN></DIV>
<DIV dir=ltr align=left>
<HR tabIndex=-1>
</DIV>
<DIV dir=ltr align=left><FONT size=2 face=Tahoma><B>From:</B>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf
Of </B>Peter Edward Prevelige Jr<BR><B>Sent:</B> Thursday, September 01, 2011
1:27 PM<BR><B>To:</B> rasmb@rasmb.bbri.org<BR><B>Subject:</B> [RASMB] Radius Out
of Range?<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV class=WordSection1>
<P class=MsoNormal>Hi All<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>I am having a host of problems trying to get a run going.
They are centered around radius problems and delay calibration. <o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Right now, I am trying to do a single scan of 3 cells at 280
nm in the 4 hole rotor, 3K RPM, 6 sector cells. I selected the option to do a
radial calibration and it is giving me radius out of range errors. I have
checked the counterbalance and it is installed properly. This is the second
counterbalance I’ve tried and as far as I know they were both working fine
previously. <o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Can anyone tell me what I might try to get this sorted
out?<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Thanks<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal>Peter<o:p></o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><o:p> </o:p></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Peter
E. Prevelige Jr.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Professor<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Dept.
of Microbiology, BBRB 416/6<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Univ.
of Alabama @ Birmingham<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">845
19th St. South<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN
style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Birmingham AL.
35294-2170<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">Phone
205 975-5327<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt">FAX
205 975-5479<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt"><A
href="mailto:prevelig@uab.edu">prevelig@uab.edu</A><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN style="FONT-FAMILY: Consolas; FONT-SIZE: 10.5pt"><A
href="http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm">http://www.microbio.uab.edu/faculty/prevelige/prevelige-p.htm</A><o:p></o:p></SPAN></P>
<P class=MsoNormal><o:p> </o:p></P></DIV></BODY></HTML>