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</o:shapelayout></xml><![endif]--></head><body lang=EN-US link=blue vlink=purple><div class=WordSection1><p class=MsoNormal>Folks,<o:p></o:p></p><p class=MsoNormal>I have a sample, a membrane protein with 0.01% DDM. I collected data at 45000 rpm, 4 min/scan. The sample is very heterogeneous but I am getting a decent peak from the absorbance data (280 nm) at 7.4 S, but the interference data gives me a peak at 6.5 S. I am trying to calculate the amount of detergent bound so I need to align the interference data with absorbance data. This is the first time in which I have gotten the peaks from the interference data not lining up with absorbance data. Is this due to the time it takes to make an absorbance scan relative to the interference scans? And/or I just spun the sample too hard? Or is the DDM affecting the sedimentation? See the attached powerpoint. <o:p></o:p></p><p class=MsoNormal>Yes, the slit assembly is functioning properly <span style='font-family:Wingdings'>J</span> <o:p></o:p></p><p class=MsoNormal>Any suggestions will be helpful! <o:p></o:p></p><p class=MsoNormal>Best,<o:p></o:p></p><p class=MsoNormal>Lake<o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal>PS I collected data during the same run with the same sample except it had GFP attached. The interference peak shifted accordingly. The DDM peak lined up well also. <o:p></o:p></p><p class=MsoNormal><o:p> </o:p></p><p class=MsoNormal><b><i><span style='font-size:10.5pt;font-family:Consolas'>Lake N. Paul, PhD<o:p></o:p></span></i></b></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>Biophysics Research Specialist<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>Discovery Park - Bindley Biosciences Center Purdue University<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>1203 West State Street<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>West Lafayette, Indiana, 47905<o:p></o:p></span></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>765.494.4960<o:p></o:p></span></p><p class=MsoNormal><b><u><span style='font-size:10.5pt;font-family:Consolas'>BioAnalytical Lab Website:<o:p></o:p></span></u></b></p><p class=MsoNormal><span style='font-size:10.5pt;font-family:Consolas'>http://www.purdue.edu/discoverypark/bioanalytical/<o:p></o:p></span></p><p class=MsoNormal><o:p> </o:p></p></div></body></html>