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<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=381545014-01042011>Sumit, the Beckman materials compatibility guide that
comes with the centrifuge (also available on their web site) lists
commercial bleach and says its use with epoxy (Epon) is satisfactory. That
list doesn't have an entry for sapphire but I'm pretty sure it can stand up
to bleach. Any detergent is okay with centerpieces and
windows.</SPAN></FONT></DIV>
<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=381545014-01042011></SPAN></FONT> </DIV>
<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=381545014-01042011>I think your biggest decontamination issue is going to
be the cell housing. During filling you almost inevitably get some
sample solution coming out around the fill holes and hence into the threads of
the housing. The aluminum housings definitely cannot stand up to
bleach. </SPAN></FONT></DIV>
<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=381545014-01042011></SPAN></FONT> </DIV>
<DIV dir=ltr align=left><FONT color=#0000ff size=2 face=Arial><SPAN
class=381545014-01042011>John</SPAN></FONT></DIV><BR>
<DIV dir=ltr lang=en-us class=OutlookMessageHeader align=left>
<HR tabIndex=-1>
<FONT size=2 face=Tahoma><B>From:</B> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf Of </B>sumit
goswami<BR><B>Sent:</B> Friday, April 01, 2011 7:27 AM<BR><B>To:</B> Brandon L
Doyle<BR><B>Cc:</B> rasmb@rasmb-email.bbri.org;
rasmb-bounces@rasmb.bbri.org<BR><B>Subject:</B> Re: [RASMB] centerpiece/window
compatibility with decontaminatingsolution<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV>Hi, </DIV>
<DIV>I guess I was wrong when I talked about using contrad for
decontaminating cell assembly. Contrad is a detergent so most likely it's not
gonna be that effective for decontamination. Bertrand mentioned about
Vikron as decontaminating agent. I will try to buy it. At this point I have
bleach solution. Does anybody know if that is okay with epoxy resin and
sapphire? </DIV>
<DIV> </DIV>
<DIV>Thank you so much!</DIV>
<DIV>Sumit<BR><BR></DIV>
<DIV class=gmail_quote>On Fri, Apr 1, 2011 at 7:51 AM, Brandon L Doyle <SPAN
dir=ltr><<A
href="mailto:DOYLE_BRANDON_L@lilly.com">DOYLE_BRANDON_L@lilly.com</A>></SPAN>
wrote:<BR>
<BLOCKQUOTE
style="BORDER-LEFT: #ccc 1px solid; MARGIN: 0px 0px 0px 0.8ex; PADDING-LEFT: 1ex"
class=gmail_quote><BR><FONT size=2 face=sans-serif>Sumit,</FONT> <BR><BR><FONT
size=2 face=sans-serif> I am not sure what variety of Contrad or
concentration you are planning on using for decontamination, but we routinely
use Contrad70 (about 0.1-1% vol/vol solutions) on Epon cells with no apparent
ill effects. We have not noticed any etching of windows (or quartz
spectroscopy cells for that matter) with those dilute conditions
either.</FONT> <BR><BR><FONT size=2 face=sans-serif>Brandon</FONT>
<BR><BR><BR><BR><BR>
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<TD width="40%"><FONT size=1 face=sans-serif><B>sumit goswami <<A
href="mailto:ikant11@gmail.com"
target=_blank>ikant11@gmail.com</A>></B> </FONT><BR><FONT size=1
face=sans-serif>Sent by: <A href="mailto:rasmb-bounces@rasmb.bbri.org"
target=_blank>rasmb-bounces@rasmb.bbri.org</A></FONT>
<P><FONT size=1 face=sans-serif>03/31/2011 10:19 PM</FONT> </P>
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<DIV align=right><FONT size=1 face=sans-serif>To</FONT></DIV>
<TD><FONT size=1 face=sans-serif><A
href="mailto:rasmb@rasmb-email.bbri.org"
target=_blank>rasmb@rasmb-email.bbri.org</A></FONT>
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<TD>
<DIV align=right><FONT size=1 face=sans-serif>cc</FONT></DIV>
<TD>
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<TD>
<DIV align=right><FONT size=1 face=sans-serif>Subject</FONT></DIV>
<TD><FONT size=1 face=sans-serif>[RASMB] centerpiece/window
compatibility with decontaminating
solution</FONT></TD></TD></TR></TD></TD></TR></TD></TD></TR></TBODY></TABLE><BR>
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<DIV></DIV>
<DIV class=h5><BR><BR><BR><FONT size=3>Hello All,</FONT> <BR><FONT
size=3>
I am planning to run some sed-vel experiments with a
protein that is cytotoxic. That means the components of the cell assembly will
require decontamination followed by the experiment. Can anyone tell me if the
centerpieces (Epon charcoal-filled) and the sapphire windows are going to
be okay with bleach or contrad? I am more concerned about the centerpieces.
</FONT><BR><FONT size=3> </FONT> <BR><FONT size=3>Thank you so
much!</FONT> <BR><FONT size=3>With regards,</FONT> <BR></DIV></DIV><FONT
size=3>Sumit</FONT><FONT
size=2><TT>_______________________________________________
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