<div>Hello All,</div>
<div> I did what Jhon suggested (HC & LC sequence merged, disulfide linkage allowed and selected N=2 for dimer) and found out the v-bar was 0.7305. Thank you so much to all of you!</div>
<div> </div>
<div>With regards,</div>
<div>Sumit<br><br></div>
<div class="gmail_quote">On Wed, Mar 23, 2011 at 7:44 AM, Mattia Rocco <span dir="ltr"><<a href="mailto:mattia.rocco@istge.it">mattia.rocco@istge.it</a>></span> wrote:<br>
<blockquote class="gmail_quote" style="PADDING-LEFT: 1ex; MARGIN: 0px 0px 0px 0.8ex; BORDER-LEFT: #ccc 1px solid"><font size="3"><br>Dear Sumit,<br><br>I second what John Philo said. In addition, we have available a program, PROMOLP, which among other things computes the vbar from the sequence, taking into account carbohydrates and phosphates. You can manually enter a sequence, or import it from a SwissProt-formatted file. I'll be glad to send it to you, or to anyone else who might want it, upon request.<br>
<br>Best - Mattia
<div class="im"><br><br>At 02:21 PM 3/22/2011 -0500, sumit goswami wrote:<br></div>
<blockquote type="cite">
<div class="im">Hello all,<br> I was trying to calculate v-bar of a mAb using SEDNTERP. I have the sequence of heavy chain (HC) and light chain (LC) seperately as expected. It seems to be little complicated. Because there are HC-HC and LC-LC disulfide linkages too.The question is how do I deal with that? Should I calculate the v-bar of Fab and Fc seperately and then calculate an weight avergae of them? <br>
<br>With regards,<br>Sumit<br></div>
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</blockquote>
<p><br>Dr. Mattia Rocco<br>Biopolimeri e Proteomica<br>Istituto Nazionale per la Ricerca sul Cancro (IST)<br>c/o Centro per le Biotecnologie Avanzate (CBA)<br>IST c/o CBA, Largo Rosanna Benzi 10<br>I-16132 Genova, Italy<br>
<br>Phone: <a href="tel:%2B39-0105737-310" target="_blank">+39-0105737-310</a><br>Fax: <a href="tel:%2B39-0105737-325" target="_blank">+39-0105737-325</a><br>e-mail: <a href="mailto:mattia.rocco@istge.it" target="_blank">mattia.rocco@istge.it</a><br>
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