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Dear Sumit,<br><br>
I second what John Philo said. In addition, we have available a program,
PROMOLP, which among other things computes the vbar from the sequence,
taking into account carbohydrates and phosphates. You can manually enter
a sequence, or import it from a SwissProt-formatted file. I'll be glad to
send it to you, or to anyone else who might want it, upon
request.<br><br>
Best - Mattia<br><br>
At 02:21 PM 3/22/2011 -0500, sumit goswami wrote:<br>
<blockquote type=cite class=cite cite>Hello all,<br>
I was trying to calculate v-bar of a mAb using SEDNTERP. I have the
sequence of heavy chain (HC) and light chain (LC) seperately as expected.
It seems to be little complicated. Because there are HC-HC and LC-LC
disulfide linkages too.The question is how do I deal with that? Should I
calculate the v-bar of Fab and Fc seperately and then calculate an weight
avergae of them? <br>
<br>
With regards,<br>
Sumit<br>
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<br>
Dr. Mattia Rocco<br>
Biopolimeri e Proteomica<br>
Istituto Nazionale per la Ricerca sul Cancro (IST)<br>
c/o Centro per le Biotecnologie Avanzate (CBA)<br>
IST c/o CBA, Largo Rosanna Benzi 10<br>
I-16132 Genova, Italy<br><br>
Phone: +39-0105737-310<br>
Fax: +39-0105737-325<br>
e-mail: mattia.rocco@istge.it<br><br>
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