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<DIV>Hi,</DIV>
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<DIV>I'm confused about which value to use in the calculation of dimerization and tetramerization constants in Sedphat.</DIV>
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<DIV>e.g. monomer-tetramer-octamer self-association model:</DIV>
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<DIV>logKa14 = 3.127, then Ka14 = 1339.677 M3, so 1/Ka14 gives 7.465 x 10-4, or 746.5 uM (which should be the equilibrium dissociation constant for the monomer : tetramer interaction).</DIV>
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<DIV>But the thermo_results file says c1 = c4 at 9.069 x 10-2 M = 90.69 mM. Is this then the tetramerization constant?</DIV>
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<DIV>I also processed the same data using monomer-n-mer self association model with n = 4:</DIV>
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<DIV>logKa1n = 3.047, then Ka1n = 1.114 x 10+3 M3, so 1/Ka1n = 4.487 x 10-4, or 448.7 uM.</DIV>
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<DIV>Again the thermo_results file says c1 = c4 at 9.642 x 10-2 M = 96.42 mM.</DIV>
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<DIV>Can someone explain please?</DIV>
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<DIV>Many Thanks,<BR><BR>Mark</DIV>
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<DIV> </DIV>
<DIV>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,<BR>Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH <BR>Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117<BR>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</DIV>
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