<HTML><HEAD>
<META http-equiv=Content-Type content="text/html; charset=utf-8">
<META content="MSHTML 6.00.6000.17063" name=GENERATOR></HEAD>
<BODY style="MARGIN: 4px 4px 1px; FONT: 10pt Tahoma">
<DIV>I checked the laser settings as advised and found:</DIV>
<DIV> </DIV>
<DIV> </DIV>
<TABLE cols=4 border=1>
<TBODY>
<TR>
<TD width=50><BR>cell</TD>
<TD width=50><BR>laser delay</TD>
<TD width=50><BR>laser duration</TD>
<TD width=50><BR>exposure time</TD></TR>
<TR>
<TD width=50>2</TD>
<TD width=50>157.1 deg<BR></TD>
<TD width=50><BR>1.7 deg</TD>
<TD width=50>6.30 uS<BR></TD></TR>
<TR>
<TD width=50>
<DIV>4<BR></DIV></TD>
<TD width=50>67.1 deg<BR></TD>
<TD width=50>0.6 deg<BR></TD>
<TD width=50>2.22 uS<BR></TD></TR>
<TR>
<TD width=50>6<BR></TD>
<TD width=50>337.6 deg<BR></TD>
<TD width=50>1.7 deg<BR></TD>
<TD width=50>6.3 uS<BR></TD></TR></TBODY></TABLE>
<DIV> </DIV>
<DIV>I also found this in the rasmb archives:</DIV>
<DIV> </DIV>
<DIV>"<SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px">The main "gotcha" about setting the duration is that if you go too high you<BR>will end up with the superposition of a single-slit interference pattern upon the<BR>double-slit interference pattern. As long as you use the typical <STRONG>0.5 - 0.8<BR>degrees for the duration</STRONG> (assuming that you are NOT using the old style interference<BR>window holders) you will be fine."</SPAN></DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"></SPAN> </DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2>So I'm thinking to bring the laser duration for cell2 and cell6 down to the value of cell4. Any further advice is greatly appreciated.</FONT></SPAN></DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2>Many Thanks,</FONT></SPAN></DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2></FONT></SPAN> </DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2>Mark</FONT></SPAN></DIV>
<DIV><SPAN class=Apple-style-span style="WORD-SPACING: 0px; FONT: medium 'Times New Roman'; TEXT-TRANSFORM: none; COLOR: rgb(0,0,0); TEXT-INDENT: 0px; WHITE-SPACE: normal; LETTER-SPACING: normal; BORDER-COLLAPSE: separate; orphans: 2; widows: 2; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px"><FONT face=Tahoma size=2></FONT></SPAN> </DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,<BR>Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH <BR>Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117<BR>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</DIV>
<DIV> </DIV>
<DIV>
<DIV>************************************************************ </DIV>
<DIV>
<DIV><FONT color=#666666>Please consider the environment. Do you really need to print this email?</FONT> </DIV></DIV><BR><BR>>>> Tom Laue <Tom.Laue@unh.edu> 2/24/2011 13:05 >>><BR>Hi Mark-<BR>The interference system requires the use of sapphire windows, except at <BR>very low rotor speeds.<BR>Modern synthetic sapphire windows may be used to 230 nm. The old <BR>sapphire windows were not clear much past 280 nm, but that is no longer <BR>an issue.<BR>I know that some labs have switched to using only sapphire window since <BR>they almost never break, and may be used with all three optical systems.<BR>Best wishes,<BR>Tom<BR><BR>On 2/24/2011 4:37 AM, Mark Agacan wrote:<BR>> Hello,<BR>> I have attached a jpeg image showing recent SV interference data plots <BR>> taken from sedfit.<BR>> All data was collected with quartz windows as I was primarily <BR>> interested in the absorbance @ 280 nm signal.<BR>> IP2 and IP6 are very noisy but IP4 looks fine.<BR>> Is this a calibration problem or is this related to damaged windows / <BR>> centerpieces (there are no visible defects or scratches on any of the <BR>> components).<BR>> Many Thanks,<BR>> Mark<BR>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>> Dr Mark Agacan, Scientific Officer for the Division of Biological <BR>> Chemistry and Drug Discovery,<BR>> Wellcome Trust Biocentre, College of Life Sciences, University of <BR>> Dundee, Dundee, DD1 5EH<BR>> Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117<BR>> ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>> ************************************************************<BR>> Please consider the environment. Do you really need to print this email?<BR>><BR>> The University of Dundee is a registered Scottish charity, No: SC015096<BR>><BR>><BR>> _______________________________________________<BR>> RASMB mailing list<BR>> RASMB@rasmb.bbri.org<BR>> <A href="http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb">http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb</A><BR><BR>-- <BR>Department of Biochemistry and Molecular Biology<BR>University of New Hampshire<BR>Durham, NH 03824-3544<BR>Phone: 603-862-2459<BR>FAX: 603-862-0031<BR>E-mail: Tom.Laue@unh.edu<BR>www.bitc.unh.edu<BR>www.camis.unh.edu<BR><BR></DIV><BR>
<p>
The University of Dundee is a registered Scottish charity, No: SC015096
</p>
</BODY></HTML>