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I disagree with John. <br>
<br>
For example, my software (SEDANAL) needs to have both the menicus and
the base positions identifiable in order to do consrevation of mass
calculations for multi-compnent systems. For an equilibrium run - since
time is not of the essentce here - is to collect enough data to include
the solution boundaries.<br>
<br>
actually, I usually collect all three channels of data in one scan and
process it afterward - once for each channel. Less muss and less fuss.<br>
<pre class="moz-signature" cols="72">
===============================================
Walter F. Stafford, Ph.D.
Senior Scientist
Boston Biomedical Research Institute
64 Grove Street
Watertown, MA 02472
617-658-7808
skype: w.stafford3
----------------------
"Be kind, for everyone you meet is fighting a hard battle." -Plato
------------------------------------ </pre>
<br>
John Philo wrote:I'll call tomorrow.<br>
<pre class="moz-signature" cols="72">
===============================================
Walter F. Stafford, Ph.D.
Senior Scientist
Boston Biomedical Research Institute
64 Grove Street
Watertown, MA 02472
617-658-7808
skype: w.stafford3
----------------------
"Be kind, for everyone you meet is fighting a hard battle." -Plato
------------------------------------ </pre>
<br>
Celia Harrison wrote:
<blockquote
cite="mid:AANLkTind+qz=1EPU0usOORARQPSOPeAX57iM70o8nzpi@mail.gmail.com"
type="cite">Hi Walter - re: my e-mail of 10 minutes ago, we found the
gif of the g(s) plot. Some lovely data, for what it's worth. Need to
move to publication quality.
<div>Can you & I talk on the phone about the data? See
recapitulated data, below.</div>
<div>Always,</div>
<div>Celia <br>
</div>
</blockquote>
<blockquote cite="mid:2F3B7B39A5F34DC49EAFF6FF5A95C2A0@79B7XD1"
type="cite">
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<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010">Lake, it seems to me for
equilibrium there is no need to capture any data to the left of the
meniscus, or even the meniscus itself. You'll just have to trim all
that region away before you analyze anyway. So the default start at 5.8
cm should capture everything you will need. On the other end I find
there is no useful (fittable) data beyond ~7.12 cm. Also remember that
UV does damage proteins, and that all three samples (3 channels) get
exposed with each flash, so at some point extra flashes to collect data
you aren't going to use does become harmful.</span></font></div>
<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010"></span></font> </div>
<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010">Roy, you may find it useful
to have both a velocity method and an equilibrium one open. I usually
do a quick velocity-mode scan to be sure everything is loaded okay and
to figure out the radial range I want, and then set up the equilibrium
method window based on the quick velocity-mode scan results.</span></font></div>
<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010"></span></font> </div>
<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010">John</span></font></div>
<div dir="ltr" align="left"><font color="#0000ff" face="Arial"
size="2"><span class="344114621-09122010"></span></font> </div>
<br>
<div dir="ltr" class="OutlookMessageHeader" align="left" lang="en-us">
<hr tabindex="-1"><font face="Tahoma" size="2"><b>From:</b>
<a class="moz-txt-link-abbreviated" href="mailto:rasmb-bounces@rasmb.bbri.org">rasmb-bounces@rasmb.bbri.org</a> [<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@rasmb.bbri.org">mailto:rasmb-bounces@rasmb.bbri.org</a>] <b>On
Behalf Of </b>Lake Paul<br>
<b>Sent:</b> Thursday, December 09, 2010 1:26 PM<br>
<b>To:</b> 'Roy Hantgan'; <a class="moz-txt-link-abbreviated" href="mailto:rasmb@rasmb.bbri.org">rasmb@rasmb.bbri.org</a><br>
<b>Subject:</b> Re: [RASMB] radial scans 6 sector cells<br>
</font><br>
</div>
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<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Roy,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Funny you
should mention this, I did my first 6-sector experiment yesterday. The
5.7 default might not cover the inside sector, so you might have to
adjust it to 5.65. I did this by trial and error and by getting help
from Dr. John Burgner. The 5.65 worked well I get alittle of the top of
the cell and both menisci. Hope this helps.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Lake<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span
style="font-family: 'Tahoma','sans-serif'; font-size: 10pt;">From:</span></b><span
style="font-family: 'Tahoma','sans-serif'; font-size: 10pt;">
<a class="moz-txt-link-abbreviated" href="mailto:rasmb-bounces@rasmb.bbri.org">rasmb-bounces@rasmb.bbri.org</a> [<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@rasmb.bbri.org">mailto:rasmb-bounces@rasmb.bbri.org</a>] <b>On
Behalf Of </b>Roy Hantgan<br>
<b>Sent:</b> Thursday, December 09, 2010 3:42 PM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:rasmb@rasmb.bbri.org">rasmb@rasmb.bbri.org</a><br>
<b>Subject:</b> [RASMB] radial scans 6 sector cells<o:p></o:p></span></p>
</div>
</div>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear colleagues:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Can you please remind me how you set the radial
distance for sed eq runs in 6-sector cells?<br>
I’ve only run sed eq in double sector cells. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks for your advice,<o:p></o:p></p>
<p class="MsoNormal">Roy <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span
style="font-family: 'Arial','sans-serif'; font-size: 10pt;">Roy R.
Hantgan, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-family: 'Arial','sans-serif'; font-size: 10pt;">Associate
Professor of Biochemistry / Associate in Molecular Medicine<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-family: 'Arial','sans-serif'; font-size: 10pt;">Director,
Macromolecular Interactions Core Laboratory (MICL)<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-family: 'Arial','sans-serif'; font-size: 10pt;">Wake
Forest University School of Medicine<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-family: 'Arial','sans-serif'; font-size: 10pt;">3058 Hanes
Bldg / TEL 336-716-4675<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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