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Hi-<br>
<br>
Whatever else you do: make sure you set the step size to 0.001 cm
and scan in "step" mode and take 4-8 replicates at each step. <br>
<br>
Walter Stafford<br>
<br>
<br>
Lake Paul wrote:
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<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Roy,<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Funny you
should mention this, I did my first 6-sector experiment yesterday. The
5.7 default might not cover the inside sector, so you might have to
adjust it to 5.65. I did this by trial and error and by getting help
from Dr. John Burgner. The 5.65 worked well I get alittle of the top of
the cell and both menisci. Hope this helps.<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);">Lake<o:p></o:p></span></p>
<p class="MsoNormal"><span style="color: rgb(31, 73, 125);"><o:p> </o:p></span></p>
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<p class="MsoNormal"><b><span
style="font-size: 10pt; font-family: "Tahoma","sans-serif";">From:</span></b><span
style="font-size: 10pt; font-family: "Tahoma","sans-serif";">
<a class="moz-txt-link-abbreviated" href="mailto:rasmb-bounces@rasmb.bbri.org">rasmb-bounces@rasmb.bbri.org</a> [<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@rasmb.bbri.org">mailto:rasmb-bounces@rasmb.bbri.org</a>] <b>On
Behalf Of </b>Roy Hantgan<br>
<b>Sent:</b> Thursday, December 09, 2010 3:42 PM<br>
<b>To:</b> <a class="moz-txt-link-abbreviated" href="mailto:rasmb@rasmb.bbri.org">rasmb@rasmb.bbri.org</a><br>
<b>Subject:</b> [RASMB] radial scans 6 sector cells<o:p></o:p></span></p>
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<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Dear colleagues:<o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Can you please remind me how you set the radial
distance for sed eq runs in 6-sector cells?<br>
I’ve only run sed eq in double sector cells. <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal">Thanks for your advice,<o:p></o:p></p>
<p class="MsoNormal">Roy <o:p></o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><span
style="font-size: 10pt; font-family: "Arial","sans-serif";">Roy R.
Hantgan, Ph.D.<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size: 10pt; font-family: "Arial","sans-serif";">Associate
Professor of Biochemistry / Associate in Molecular Medicine<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size: 10pt; font-family: "Arial","sans-serif";">Director,
Macromolecular Interactions Core Laboratory (MICL)<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size: 10pt; font-family: "Arial","sans-serif";">Wake
Forest University School of Medicine<o:p></o:p></span></p>
<p class="MsoNormal"><span
style="font-size: 10pt; font-family: "Arial","sans-serif";">3058 Hanes
Bldg / TEL 336-716-4675<o:p></o:p></span></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
<p class="MsoNormal"><o:p> </o:p></p>
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<br>
<pre class="moz-signature" cols="72">--
===============================================
Walter F. Stafford, Ph.D.
Senior Scientist
Boston Biomedical Research Institute
64 Grove Street
Watertown, MA 02472
617-658-7808
skype: w.stafford3
----------------------
"Be kind, for everyone you meet is fighting a hard battle." -Plato
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