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<DIV>Hello,</DIV>
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<DIV>I'm trying to run a sedimentation equilibrium experiment at 3 wavelengths. W1 is 280 nm , W2 is 250 nm and W3 is 230 nm. The first scan completes ok at W1, but at the second wavelength the software crashes just before it completes the scan. </DIV>
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<DIV>I've heard that this could be due to the software version I'm using, v.4.5.</DIV>
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<DIV>Other parameters are:</DIV>
<DIV>Step mode; 20 replicates; 0.001 radial step size; 6-channel centerpiece; 8-hole rotor; one cell in position 4, counterbalance in position 8; Acquiring intensity instead of OD data; Absorbance scans only; 10 000 rpm;</DIV>
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<DIV>Has anyone had similar problems and found a remedy?</DIV>
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<DIV>Many Thanks,</DIV>
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<DIV>Mark</DIV>
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<DIV>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and Drug Discovery,<BR>Wellcome Trust Biocentre, College of Life Sciences, University of Dundee, Dundee, DD1 5EH <BR>Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117<BR>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</DIV>
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