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<DIV dir=ltr align=left><SPAN class=025344115-16092010>Mark, I have not had
similar problems when using multiple wavelengths for absorbance, so I am
going to guess that this is a software bug related to trying to save these scans
as intensity rather than absorbance. You could test that theory pretty quickly
if you also drop the replicates and up the radial spacing.</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=025344115-16092010></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=025344115-16092010>In general I would
recommend filling the cells in a pattern that minimizes the number of wavelength
changes. That is, I typically will group samples at similar concentrations
together in the same cell so fewer changes are needed.</SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=025344115-16092010></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=025344115-16092010>John</SPAN></DIV><BR>
<DIV dir=ltr lang=en-us class=OutlookMessageHeader align=left>
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<B>From:</B> rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org]
<B>On Behalf Of </B>Mark Agacan<BR><B>Sent:</B> Thursday, September 16, 2010
7:33 AM<BR><B>To:</B> rasmb@server1.bbri.org<BR><B>Subject:</B> [RASMB]
Multi-wavelength SE crash<BR><BR></DIV>
<DIV></DIV>
<DIV>Hello,</DIV>
<DIV> </DIV>
<DIV>I'm trying to run a sedimentation equilibrium experiment at 3
wavelengths. W1 is 280 nm , W2 is 250 nm and W3 is 230 nm. The
first scan completes ok at W1, but at the second wavelength the
software crashes just before it completes the scan. </DIV>
<DIV> </DIV>
<DIV>I've heard that this could be due to the software version I'm using,
v.4.5.</DIV>
<DIV> </DIV>
<DIV>Other parameters are:</DIV>
<DIV>Step mode; 20 replicates; 0.001 radial step size; 6-channel centerpiece;
8-hole rotor; one cell in position 4, counterbalance in position 8; Acquiring
intensity instead of OD data; Absorbance scans only; 10 000 rpm;</DIV>
<DIV> </DIV>
<DIV>Has anyone had similar problems and found a remedy?</DIV>
<DIV> </DIV>
<DIV>Many Thanks,</DIV>
<DIV> </DIV>
<DIV>Mark</DIV>
<DIV> </DIV>
<DIV> </DIV>
<DIV>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<BR>Dr Mark
Agacan, Scientific Officer for the Division of Biological Chemistry and Drug
Discovery,<BR>Wellcome Trust Biocentre, College of Life Sciences, University of
Dundee, Dundee, DD1 5EH <BR>Tel: +44 1382 386095 Fax: +44 1382
345764 Mobile: 07525 451
117<BR>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~</DIV>
<DIV> </DIV>
<DIV> </DIV><BR>
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