<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Dave,<div><br></div><div>I'm no expert, but wouldn't DSC address this? Also, I haven't studied up on this in a while, but Brian Matthews' lab did some research on void filling in lysozyme. There might be some answers therein.</div><div><br></div><div>Chad</div><div><br></div><div><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; "><span class="Apple-style-span" style="border-collapse: separate; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; -webkit-text-decorations-in-effect: none; text-indent: 0px; -webkit-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div>======================================</div><div>Chad A. Brautigam, Ph.D.</div><div>Assistant Professor</div><div>Department of Biochemistry</div><div>The University of Texas</div><div>Southwestern Medical Center at Dallas</div><div>Department of Biochemistry</div><div>5323 Harry Hines Blvd.</div><div>Rm. ND10.214A</div><div>Dallas, TX 75390-8816</div><div>Office: 214-645-6384</div><div>Fax: 214-645-6353</div><div>Email: <a href="mailto:chad.brautigam@utsouthwestern.edu">chad.brautigam@utsouthwestern.edu</a></div><div><br class="khtml-block-placeholder"></div><br class="Apple-interchange-newline"></span></div></span></div></span><br class="Apple-interchange-newline"></div></span><br class="Apple-interchange-newline"> </div><br><div><div>On Jun 3, 2010, at 11:08 AM, Dj Scott wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Helvetica; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><div lang="EN-GB" link="blue" vlink="purple"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="country-region"><o:smarttagtype namespaceuri="urn:schemas-microsoft-com:office:smarttags" name="place"><div class="Section1"><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Hello all in RASMB-land.<o:p></o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">A question for you.<o:p></o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; "><o:p> </o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">If I make a void in a protein by mutating a larger residue to a smaller one in the hydrophobic core, what is the thermodynamic cost of this, and how would it be best to measure it.<o:p></o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; "><o:p> </o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">All thoughts welcome.<o:p></o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Best<o:p></o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; "><o:p> </o:p></span></font></div><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Dave.<o:p></o:p></span></font></div><p style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; "><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">>>>>>>>>>>>>>>>>>>>>>>>>>></span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Dr. David J. Scott</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Associate Professor in Physical Biochemistry</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">National Centre for Macromolecular Hydrodynamics</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">School of Biosciences</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">University of Nottingham</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Sutton Bonington Campus</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Leicestershire. LE12 5RD.</span></font><span class="Apple-converted-space"> </span><br><st1:country-region w:st="on"><st1:place w:st="on"><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">United Kingdom</span></font></st1:place></st1:country-region><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">.</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Phone: +44 (0) 115 951 6221</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Fax: +44 (0) 115 951 6142</span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Email:<span class="Apple-converted-space"> </span><a href="mailto:dj.scott@nottingham.ac.uk" style="color: blue; text-decoration: underline; ">dj.scott@nottingham.ac.uk</a></span></font><span class="Apple-converted-space"> </span><br><font size="2" face="Georgia"><span style="font-size: 10pt; font-family: Georgia; ">Web:<span class="Apple-converted-space"> </span><a href="http://www.nottingham.ac.uk/ncmh" style="color: blue; text-decoration: underline; ">www.nottingham.ac.uk/ncmh</a></span></font><o:p></o:p></p><div style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; margin-top: 0cm; margin-bottom: 0.0001pt; "><font size="3" face="Times New Roman"><span style="font-size: 12pt; "><o:p> </o:p></span></font></div></div><br><p style="margin-right: 0cm; margin-left: 0cm; font-size: 12pt; font-family: 'Times New Roman'; ">This message has been checked for viruses but the contents of an attachment may still contain software viruses which could damage your computer system: you are advised to perform your own checks. Email communications with the University of Nottingham may be monitored as permitted by UK legislation.</p>_______________________________________________<br>RASMB mailing list<br><a href="mailto:RASMB@rasmb.bbri.org" style="color: blue; text-decoration: underline; ">RASMB@rasmb.bbri.org</a><br><a href="http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb" style="color: blue; text-decoration: underline; ">http://rasmb.bbri.org/cgi-bin/mailman/listinfo/rasmb</a><br></o:smarttagtype></o:smarttagtype></div></span></blockquote></div><br></div></body></html>