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<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Hi Arne,<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>I will let more knowledgeable people give
possible reasons for the dark fringe patterns (Walter Stafford once told me an
XLI was once focused on the second order fringes instead of the first order fringes,
but I think he saw what that looked like and I only heard about it), but as a
lab with an XLI fully upgraded to Proteome Lab version, I wanted to say that
our interference system works better than what is seen in your screen shots. <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>In older systems there was a brightness
and contrast setting, but in newer systems there is no brightness or contrast
control: it is supposed to be automatic. My XLI has the same laser
setting screen as in your screen shot (no brightness or contrast control). However,
for standard double sector cells without interference slits on the top window,
my laser duration is set to 0.1 degree. I can use 0.2 degrees, but the
fringes look too bright and washed out. My fringes would be completely
oversaturated at 0.6 degrees duration which is on your screen shot. With
the thin slit window holders (sold by Spin Analytical) my duration is set to
1.2 degrees since the slits control the light intensity and the brightness of
the fringes is always a little too bright by my judgment and I wish I still had
the contrast/brightness controls.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>Our friendly Beckman maintenance man, Sam
Fishpaw, would never leave a PM visit with fringes looking like this.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'>David Hayes<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
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<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <b><span
style='font-weight:bold'>On Behalf Of </span></b>Rufer, Arne<br>
<b><span style='font-weight:bold'>Sent:</span></b> Thursday, April 29, 2010
9:35 AM<br>
<b><span style='font-weight:bold'>To:</span></b> rasmb@rasmb.bbri.org<br>
<b><span style='font-weight:bold'>Subject:</span></b> [RASMB] Dark interference
fringe pattern</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Dear colleagues,</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>we recently encounter problems with the interference optical
system on our XLI (upgraded to Proteome Lab version) in that the fringe pattern
looks very dark (please see attached screenshots). Beckman field service
recommended to us to clean the lenses in the laser housing of the monochomator
arm (which we did paying attention to reinstall the lens in the correct
orientation) and to clean/exchange the filter above the condensor lens.
While intermittantly the brightness/contrast of the fringe pattern improved, we
are back to "very dark" after trying to further clean things up. What
remains also is a concentric interference pattern superimposed to the expected
fringes, which you can see on the left side of the fringe patttern in the
second screen shot. This ghost pattern is also visible when we look at the
scallop position or an empty cell hole.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Any suggestion as to how we can get our machine back to
normal would be greatly apprecieated.</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
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<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Best regards,</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Arne</span></font><o:p></o:p></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
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<div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
</div>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Dr. Arne Rufer</span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>F. Hoffmann-La Roche AG</span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>PROBMO Optical Spectroscopy</span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>065/207</span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>CH-4070 <st1:City w:st="on"><st1:place w:st="on">Basel</st1:place></st1:City></span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Tel: 0041-(0)61-6882126</span></font><o:p></o:p></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><a href="mailto:arne.rufer@roche.com">arne.rufer@roche.com</a></span></font><o:p></o:p></p>
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<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'> <o:p></o:p></span></font></p>
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