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<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Good morning, <o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Just out of curiosity:<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>One can measure rotor stretching that occurs already at 3000
rpm. So I wonder whether imbalance also causes wobbling/tilting of the rotor at
low speed? And if so, wouldn’t that impact the calibration procedure? <o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Is there any disadvantage performing the optical checks/calibrations
on an empty hole with a rotor with balanced centerpiece and cell? <o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Best<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Titus<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><o:p> </o:p></span></p>
<p class=MsoNormal><span lang=DE style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><o:p> </o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Titus M. Franzmann, Dr. <o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Research Fellow<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>University of Michigan<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Mol. Cell. and Develop. Biol.<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>4140C Nat. Sci. Bldg<o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>830 N. University Ave <o:p></o:p></span></p>
<p class=MsoNormal><span lang=DE style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>Ann Arbor, Mi, 48109-1048, USA<o:p></o:p></span></p>
<p class=MsoNormal><span lang=DE style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'>+1 (734) 647 2291<o:p></o:p></span></p>
<p class=MsoNormal><span lang=DE style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><a href="mailto:tmfr@umich.edu"
title="send an email to tmfr@umich.edu"><span style='color:blue'>tmfr@umich.edu</span></a><o:p></o:p></span></p>
<p class=MsoNormal><span lang=DE style='font-size:8.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><a href="http://www.mcdb.lsa.umich.edu/labs/walter/"
title="click to visit the walter lab web sites"><span style='color:blue'>walter
lab website</span></a><o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><o:p> </o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><o:p> </o:p></span></p>
<p class=MsoNormal><span style='font-size:11.0pt;font-family:"Segoe UI","sans-serif";
color:#1F497D'><o:p> </o:p></span></p>
<div>
<div style='border:none;border-top:solid #B5C4DF 1.0pt;padding:3.0pt 0cm 0cm 0cm'>
<p class=MsoNormal><b><span lang=DE style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Von:</span></b><span
lang=DE style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>
rasmb-bounces@server1.bbri.org [mailto:rasmb-bounces@server1.bbri.org] <b>Im
Auftrag von </b>Karel Planken<br>
<b>Gesendet:</b> Mittwoch, 17. Februar 2010 06:43<br>
<b>An:</b> Mark Agacan; rasmb@server1.bbri.org<br>
<b>Betreff:</b> Re: [RASMB] Xl-I wavelength check question<o:p></o:p></span></p>
</div>
</div>
<p class=MsoNormal><o:p> </o:p></p>
<p class=MsoNormal>Dear Mark,<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
<p class=MsoNormal>Just measure through a windowless cell (complete cell
without windows). The weight difference with the counterbalance may be up to 5
g but as long as the rotor is not spinning above 3000 rpm you're safe. You may
want to look up the appendix in the hardware manual available
at "<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><a
href="http://ultrascan.uthscsa.edu/aucmanual/AUCuserGuideVolume-1-Hardware.pdf"><span
style='font-size:12.0pt;font-family:"Times New Roman","serif";color:black'>http://ultrascan.uthscsa.edu/aucmanual/AUCuserGuideVolume-1-Hardware.pdf</span></a></span>".
Good luck,<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
<p class=MsoNormal>Karel Planken<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
<p class=MsoNormal>Dr. K.L. Planken<span style='font-size:10.0pt;font-family:
"Tahoma","sans-serif"'><o:p></o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<p class=MsoNormal>Max-Planck-Institute of Colloids and Interfaces<br>
Colloid Chemistry<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<p class=MsoNormal>Am Mühlenberg 1<br>
D-14476 Golm<br>
Germany<span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<p class=MsoNormal>Tel.: +49 331 567 9518<br>
Fax: +49 331 567 9502<br>
Office:K-2.102<br>
E-mail: <a href="mailto:karel.planken@mpikg.mpg.de">karel.planken@mpikg.mpg.de</a>..<span
style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p></o:p></span></p>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p> </o:p></span></p>
<div class=MsoNormal align=center style='text-align:center'><span
style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>
<hr size=2 width="100%" align=center>
</span></div>
<p class=MsoNormal style='margin-bottom:12.0pt'><b><span style='font-size:10.0pt;
font-family:"Tahoma","sans-serif"'>From:</span></b><span style='font-size:10.0pt;
font-family:"Tahoma","sans-serif"'> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <b>On Behalf Of </b>Mark Agacan<br>
<b>Sent:</b> Wednesday, 17. February 2010 11:49<br>
<b>To:</b> rasmb@server1.bbri.org<br>
<b>Subject:</b> [RASMB] Xl-I wavelength check question<o:p></o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>I
have a question regarding the wavelength accuracy check for the XL-I:<o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Using
a windowless cell (aluminium cell housing only with no centerpiece or windows,
or one of the empty holes in the rotor?) - how
can I match the weight of this cell with the counterbalance, even at
the relatively low speed of 3000 rpm?<o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>The
remainder of the procedure as outlined in the Troubleshooting and Maintenance
section of the XL-I manual seems straighforward enough, i'm just a little
unclear on exactly how to load the rotor for this measurement.<o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Best
Regards<o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>Mark<o:p></o:p></span></p>
</div>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'> <o:p></o:p></span></p>
</div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'><o:p> </o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<br>
Dr Mark Agacan, Scientific Officer for the Division of Biological Chemistry and
Drug Discovery,<br>
Wellcome Trust Biocentre, College of Life Sciences, University of Dundee,
Dundee, DD1 5EH <br>
Tel: +44 1382 386095 Fax: +44 1382 345764 Mobile: 07525 451 117<br>
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~<o:p></o:p></span></p>
</div>
<p class=MsoNormal style='margin-bottom:12.0pt'><span style='font-size:10.0pt;
font-family:"Tahoma","sans-serif"'><br>
<br>
<o:p></o:p></span></p>
<div>
<p class=MsoNormal><span style='font-size:10.0pt;font-family:"Tahoma","sans-serif"'>************************************************************<br>
<span style='color:#666666'>Please consider the environment. Do you really need
to print this email?</span> <o:p></o:p></span></p>
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