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<TITLE>Re: [RASMB] Query concerning membrane protein sedimentation equilibrium experiment</TITLE>
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Hi John (and everyone)<BR>
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That sounds good to me. Of course you could have more than one protein monomer contained in each micelle - but that should be immediately obvious from the M value.<BR>
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Kind regards<BR>
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Arthur<BR>
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Arthur J Rowe<BR>
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University of Nottingham<BR>
School of Biosciences<BR>
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<BLOCKQUOTE><B>From: </B>John_Doran@vrtx.com<BR>
<B>Date: </B>Mon, 2 Nov 2009 16:46:47 -0500<BR>
<B>To: </B>rasmb@rasmb.bbri.org<BR>
<B>Subject: </B>[RASMB] Query concerning membrane protein sedimentation equilibrium experiment<BR>
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<FONT SIZE="2">We have measured the amount of detergent bound to our membrane protein and hence were able to calculate a Vbar for the protein detergent complex. Is it sufficient to just substitute this value for Vbar along with the density value of the detergent buffer solution into the ideal species equation of heteroanalysis to get an accurate molecular weight value of the protein-detergent complex, or is it more involved than that? Any thoughts concerning this would be appreciated. Thank you.</FONT> <BR>
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<FONT SIZE="2">John Doran</FONT> <BR>
<FONT SIZE="2">Vertex Pharmaceuticals</FONT> <BR>
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