<html><body style="word-wrap: break-word; -webkit-nbsp-mode: space; -webkit-line-break: after-white-space; ">Hi Holger,<div><br></div><div><span class="Apple-tab-span" style="white-space:pre"> </span>SEDANAL will handle floatation by specifying a negative sedimentation coefficient for the floating species. You will need to allow a negative value for s in the model editor as well as on the control screen.</div><div><br></div><div>Try it out first in the simulator.</div><div><br></div><div>Walter<br><div apple-content-edited="true"> <span class="Apple-style-span" style="border-collapse: separate; color: rgb(0, 0, 0); font-family: Century; font-size: medium; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; orphans: 2; text-align: auto; text-indent: 0px; text-transform: none; white-space: normal; widows: 2; word-spacing: 0px; -webkit-border-horizontal-spacing: 0px; -webkit-border-vertical-spacing: 0px; -webkit-text-decorations-in-effect: none; -webkit-text-size-adjust: auto; -webkit-text-stroke-width: 0px; "><span class="Apple-style-span" style="font-family: Helvetica; font-size: 12px; "><div><br class="Apple-interchange-newline"><br></div><div>Walter F. Stafford, Ph.D.</div><div>Senior Scientist</div><div>Boston Biomedical Research Institute</div><div>64 Grove Street</div><div>Watertown, MA 02472</div><div>617-658-7808</div><div><a href="mailto:stafford@bbri.org">stafford@bbri.org</a></div><div><br class="webkit-block-placeholder"></div><br class="Apple-interchange-newline"><br class="Apple-interchange-newline"></span></span> </div><br><div><div>On Aug 4, 2009, at 04:29, Holger Strauss wrote:</div><br class="Apple-interchange-newline"><blockquote type="cite"><div>Hello everybody,<br><br>recently, a sample finally found its way to me that has floating AND sedimenting (macromolecular) components. Both are detected and it is important to characterize both, at least their partial concentrations. So.... did I mention already that both fractions are polydisperse?<br><br>As far as I'm aware, no Lamm-equation based algorithm will calculate a distribution going from negative to positive. Please do correct me if I'm wrong!<br>I've performed synthetic boundary runs, so at least I have data with upper and lower plateaus. Any suggestions on how to treat these data in a model-independent way, i.e. where to put the (permeable) meniscus? (Walter, my hopes are somewhat resting on Sedanal...?)<br>Have the ancient masters encountered anything like that before? How did they deal with it?<br><br>Best wishes to all of you,<br>Holger<br><br>-- <br>Nanolytics<br>Gesellschaft fuer Kolloidanalytik mbH<br>Dr. Holger Strauss<br><br>Am Muehlenberg 11<br>D-14476 Potsdam<br>Tel: +49 331 5818360<br>Fax: +49 331 5818361<br>e-mail:<span class="Apple-tab-span" style="white-space:pre"> </span><a href="mailto:strauss@nanolytics.de">strauss@nanolytics.de</a><br>Internet: www.nanolytics.de<br>_______________________________________________<br>Diese E-Mail kann vertrauliche und/oder rechtlich geschützte Informationen enthalten. Wenn Sie nicht der richtige Adressat sind oder diese E-Mail irrtümlich erhalten haben, informieren Sie bitte sofort den Absender und vernichten Sie diese Mail. Das unerlaubte Kopieren sowie die unbefugte Weitergabe dieser Mail ist nicht gestattet.<br><br>This e-mail may contain confidential and/or privileged information. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly forbidden.<br><br>_______________________________________________<br>RASMB mailing list<br>RASMB@rasmb.bbri.org<br>http://rasmb.bbri.org/mailman/listinfo/rasmb<br><br></div></blockquote></div><br></div></body></html>