<HTML><HEAD>
<META http-equiv=Content-Type content="text/html; charset=iso-8859-15">
<META content="MSHTML 6.00.6000.16809" name=GENERATOR></HEAD>
<BODY style="MARGIN: 4px 4px 1px; FONT: 10pt Tahoma">
<DIV>Sednterp has an option to calculate vbar in the presence of urea or guanidine. This is probably based upon work primarily from Timasheff & coworkers. As a start see reviews in Methods Enzymol vol 61: chapter 3) Lee et al, chapter 4) Lee & Timasheff. Also Prakash & Timasheff, Anal Biochem 117, 330 (1981). Prakash et al ABB 210, 455 (1981).</DIV>
<DIV> </DIV>
<DIV>As to intermediates there is some AUC data on this - maybe more with detergents but some data as a function of denaturant concentration - but you are now really in the realm of folders and need to ask a different audience about ensembles vs intermediates.</DIV>
<DIV> </DIV>
<DIV> <BR><BR>>>> On 3/28/2009 at 7:51 AM, in message <020501c9afa3$e216b9c0$a6442d40$@edu>, "Titus M. Franzmann" <tmfr@umich.edu> wrote:<BR></DIV>
<DIV style="PADDING-LEFT: 7px; MARGIN: 0px 0px 0px 15px; BORDER-LEFT: #050505 1px solid; BACKGROUND-COLOR: #f3f3f3">
<DIV class=Section1>
<P class=MsoNormal><SPAN lang=EN-US>Hello to everyone,<?xml:namespace prefix = o ns = "urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Is there information available about how vbar changes during chemical induced protein unfolding / refolding, or how to assess the population of differently folded intermediates.<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Thank you very much for your input. <o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Best wishes<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Titus<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US><o:p> </o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Titus M. Franzmann<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>University of Michigan<o:p></o:p></SPAN></P>
<P class=MsoNormal><SPAN lang=EN-US>Molecular, Cellular, Developmental and Biology Department (MCDB)<o:p></o:p></SPAN></P>
<P class=MsoNormal>830 North University<o:p></o:p></P>
<P class=MsoNormal>Ann Arbor, Mi, 48109, USA<o:p></o:p></P></DIV></DIV><br clear=all> Individuals who have received this information in error or are not authorized to receive it must promptly return or dispose of the information and notify the sender. Those individuals are hereby notified that they are strictly prohibited from reviewing, forwarding, printing, copying, distributing or using this information in any way.
</BODY></HTML>