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<TITLE>Re: [RASMB] AUC of protein formulation</TITLE>
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</BLOCKQUOTE><FONT COLOR="#800000">Hi John<BR>
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This is an area where I do quite a bit of work, as you may guess. My first thought is "why do you need to know the solvent parameters, given that what you are looking for is simply degree of oligomerisation?". The lack of such knowledge may indeed slightly mess up the absolute values in a c(M) profile, but given that you almost certainly know the formula weight of your monomeric product, who cares? What you get will surely be good enough to say "that peak is the monomer!"<BR>
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However, a second thought comes to mind, again based upon experience, when I see that you have "surface-active excipients" present. These may well form micellar structures in solution, into which the proteins monomers etc are incorporated. But then again, the amount of such excipients added may be minimal, and below the CMC (critical micelle concentration). There are ways of handling such systems, and I have recently published some novel approaches which might be applicable in your case. I will happily enlarge on this topic if it seems relevant to your system.<BR>
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Kind regards<BR>
<BR>
Arthur<BR>
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--<BR>
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Arthur J Rowe<BR>
Professor of Biomolecular Technology<BR>
NCMH Business Centre<BR>
University of Nottingham<BR>
School of Biosciences<BR>
Sutton Bonington<BR>
Leicestershire LE12 5RD UK<BR>
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Tel: +44 (0)115 951 6156<BR>
+44 (0)116 271 4502<BR>
Fax: +44 (0)115 951 6157<BR>
email: arthur.rowe@nottingham.ac.uk<BR>
Web: www.nottingham.ac.uk/ncmh/business<BR>
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<BLOCKQUOTE><B>From: </B>john.b.babetas@gskbio.com<BR>
<B>Date: </B>Mon, 3 Nov 2008 09:12:33 -0500<BR>
<B>To: </B>rasmb@rasmb.bbri.org<BR>
<B>Subject: </B>[RASMB] AUC of protein formulation<BR>
<BR>
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<FONT SIZE="2">To whom it may concern,</FONT> <BR>
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<FONT SIZE="2">I am in the process of using Sedimentation Velocity- Analytical Ultracentrifugation to evaluate the aggregation of a protein formulation. The formulation is complex containing Sugars, Monothioglycerol and surfactant excipients. I wanted to know what needs to be done in terms of Data processing to deal with a complex matrix as this one. When i use sednterp not all the components of the solvent are there. So i cannot determine the density and viscosity with exactitude. I know that the inhomogeneous solvent option deals with dynamic gradients coming from one co-solute and not many. I wanted to know what are my options to overcome this problem.</FONT> <BR>
<BR>
John<BR>
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