<html xmlns:m="http://schemas.microsoft.com/office/2004/12/omml">
<head>
<META HTTP-EQUIV="Content-Type" CONTENT="text/html; charset=iso-8859-1">
<meta name=Generator content="Microsoft Word 10 (filtered)">
<style>
<!--a:link
{mso-style-priority:99;}
span.MSOHYPERLINK
{mso-style-priority:99;}
a:visited
{mso-style-priority:99;}
span.MSOHYPERLINKFOLLOWED
{mso-style-priority:99;}
/* Font Definitions */
@font-face
{font-family:Tahoma;
panose-1:2 11 6 4 3 5 4 4 2 4;}
@font-face
{font-family:Calibri;}
/* Style Definitions */
p.MsoNormal, li.MsoNormal, div.MsoNormal
{margin:0cm;
margin-bottom:.0001pt;
font-size:11.0pt;
font-family:Calibri;}
a:link, span.MsoHyperlink
{color:blue;
text-decoration:underline;}
a:visited, span.MsoHyperlinkFollowed
{color:purple;
text-decoration:underline;}
span.StyleCourrierlectronique17
{font-family:Calibri;
color:windowtext;}
span.StyleCourrierlectronique18
{font-family:Arial;
color:navy;}
@page Section1
{size:612.0pt 792.0pt;
margin:72.0pt 72.0pt 72.0pt 72.0pt;}
div.Section1
{page:Section1;}
-->
</style>
</head>
<body lang=FR link=blue vlink=purple>
<div class=Section1>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>Dear Joris, dear all, </span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>This reference may be
perhaps useful.</span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'> </span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=DE
style='font-size:10.0pt;font-family:Arial;color:blue'>Solovyova A., Schuck P.,
Costenaro L., Ebel C,</span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>Non-ideality by
sedimentation velocity of halophilic malate dehydrogenase in complex solvents, </span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>(2001) <i><span
style='font-style:italic'>Biophys. J,</span></i> 81 1868-1880.</span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'> </span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>In this work, we analyzed
sedimentation velocity profiles considering hydrodynamic and thermodynamic non ideality.
(i.e. concentration dependency of s and D) in the case of an homogeneous solution
of our protein of interest. The modified Lamm equation was implemented in a
model of analysis in Sedfit (note that, unless the programme was recently
modified, the kS and kD are expressed in signal unit in sedfit)</span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>From my experience, the
sedimentation velocity profiles are essentially modified by the concentration
dependency of the sedimentation coefficient. Thus the concentration dependency
of D can be neglected in a first approximation. Also from a theoretical point
of view, kD is much lower that ks. </span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>All the best</span></font></p>
<p class=MsoNormal><font size=2 color=blue face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:blue'>Christine </span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span lang=EN-GB
style='font-size:10.0pt;font-family:Arial;color:navy'> </span></font></p>
<div>
<p class=MsoNormal><font size=2 color=navy face="Courier New"><span lang=EN-GB
style='font-size:10.0pt;font-family:"Courier New";color:navy'>Christine EBEL</span></font></p>
<p class=MsoNormal><font size=2 color=navy face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New";color:navy'>Institut de
Biologie Structurale CEA-CNRS-UJF</span></font></p>
<p class=MsoNormal><font size=2 color=navy face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New";color:navy'>41 rue Jules
Horowitz, F-38027 Grenoble France</span></font></p>
<p class=MsoNormal><font size=2 color=navy face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New";color:navy'>Tel (33) (0) 4 38
78 95 70; Fax (33) (0) 4 38 78 54 94 </span></font></p>
<p class=MsoNormal><font size=2 color=navy face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New";color:navy'>christine.ebel@ibs.fr</span></font></p>
<p class=MsoNormal><font size=2 face=Calibri><span style='font-size:11.0pt'><a
href="http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm"><font
size=2 color=navy face="Courier New"><span style='font-size:10.0pt;font-family:
"Courier New";color:navy'>http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm</span></font></a></span></font></p>
</div>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'>-----Message d'origine-----<br>
<b><span style='font-weight:bold'>De :</span></b>
rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <b><span
style='font-weight:bold'>De la part de</span></b> Beld, Joris<br>
<b><span style='font-weight:bold'>Envoyé :</span></b> lundi 1 septembre
2008 15:24<br>
<b><span style='font-weight:bold'>À :</span></b> RASMB@rasmb.bbri.org<br>
<b><span style='font-weight:bold'>Objet :</span></b> [RASMB] upper
concentration limit AUC</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
style='font-size:11.0pt'> </span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Dear all,</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'> </span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>A colleague asked me whether analytical
ultracentrifugation has an upper limit with regard to the concentration of the
protein. They want to measure the protein at the same concentration as the NMR
experiments (> 1 mM). I am not entirely sure but I thought this should be no
problem. One could easily measure off-peak at another wavelength than 230nm,
e.g. 235nm or 280nm, right?! Or does one run into non-ideality phenomena when
doing sedimentation equilibrium at these high protein concentrations?!</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Thanks a lot in advance for any feedback.</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Best wishes,</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'> </span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Joris Beld</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'> </span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Hilvert Group</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>ETH Zürich</span></font></p>
<p class=MsoNormal style='margin-left:35.4pt'><font size=2 face=Calibri><span
lang=EN-US style='font-size:11.0pt'>Switzerland</span></font></p>
</div>
</body>
</html>