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<DIV><FONT face=Arial size=2>Dear Christine and Colleagues,</FONT></DIV>
<DIV><FONT face=Arial size=2>Thanks Christine for attaching the
Anal.Biochem paper 334 (2004) as contribution to clearify and
support (page332),</FONT></DIV>
<DIV><FONT face=Arial size=2>a mode to achieve the partial specific
volume from<FONT size=3><STRONG>S</STRONG></FONT><FONT size=1><SPAN><SPAN
style="mso-char-type: symbol; mso-symbol-font-family: 'WP Greek Courier'">eta</SPAN> </SPAN></FONT><SPAN><STRONG><FONT
size=3> </STRONG><FONT size=2>versus</FONT><STRONG> ρ</STRONG> <FONT size=1>of
H<SPAN>2O and D2O </SPAN></FONT><SPAN lang=EN-GB
style="FONT-FAMILY: Arial; mso-ansi-language: EN-GB"><FONT
size=1>buffer</FONT><FONT size=2> </SPAN>for heterogenous samples, so
sure also for non-heterogenous samples,( in my case
protein-nucleic- acids- complexes of about 70S' and 4 million Dalton
that I cold in my latest mail( 8
June2008) biological samples. The knowledge how to achieve
the Mw's of those complexex are more and
more required, and they are not always discribed in the
latest books about AUC. One has to dig in old
papers of the 60-</FONT></FONT></SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN><FONT size=3>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="FONT-FAMILY: Arial; mso-ansi-language: EN-GB"><FONT size=2><SPAN>80teens
as my reference of C.H: Huang at al.(JBC </SPAN>1971 Vol.
216)......ariel</FONT></SPAN></P></FONT></SPAN></FONT></DIV>
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