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Dear All,<div><br></div><div>Much to my dismay, I am forced to conclude that one of my centerpiece/window combinations for our analytical ultracentrifuge has become contaminated with some evil microbe. How do I know? Well, whatever's in there, it's tearing apart both protein and RNA with gleeful abandon. On Monday, I had a 27 kDa protein reduced to amino acids in mere hours. Terrifying!</div><div><br></div><div>So I'm wondering how people have defeated these enemies in the past. I see in the archive that RNAseZap is recommended for killing the RNAses, but I didn't come across anything to kill the hungry bugs. A little fire would teach them a lesson, but I doubt the centerpiece would tolerate that very well. I see from the chemical compatibility charts that I could go after the evildoers with acetic (glacial!) or hydrochloric acids. Any suggestions are welcome and appreciated.</div><div><br></div><div>And please, do not hold back! I am willing to get extremely medieval on their microscopic butts. Remember, this isn't about good science anymore- it's about revenge.</div><div><br></div><div>With anticipation,</div><div>Chad</div><div><br><div> <span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><span class="Apple-style-span" style="border-collapse: separate; border-spacing: 0px 0px; color: rgb(0, 0, 0); font-family: Helvetica; font-size: 12px; font-style: normal; font-variant: normal; font-weight: normal; letter-spacing: normal; line-height: normal; text-align: auto; -khtml-text-decorations-in-effect: none; text-indent: 0px; -apple-text-size-adjust: auto; text-transform: none; orphans: 2; white-space: normal; widows: 2; word-spacing: 0px; "><div>======================================</div><div>Chad A. Brautigam, Ph.D.</div><div>Assistant Professor</div><div>Department of Biochemistry</div><div>The University of Texas</div><div>Southwestern Medical Center at Dallas</div><div>Department of Biochemistry</div><div>5323 Harry Hines Blvd.</div><div>Dallas, TX 75390-8816</div><div>Office: 214-645-6384</div><div>Fax: 214-645-6353</div><div>Email: <a href="mailto:chad.brautigam@utsouthwestern.edu">chad.brautigam@utsouthwestern.edu</a></div><div><br class="khtml-block-placeholder"></div><br class="Apple-interchange-newline"></span></span> </div><br></div></body></html>