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<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Hello everyone,<o:p></o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>I am very new in the DSC
field and I have a few questions that I thought someone here will be able to
help me.<o:p></o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>1) Has anyone run DSC with
Manitol in the formulation buffer? Are there any potential problems associated
with manitol? <o:p></o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>2) Has anyone run DSC of
pegylated protein? Do you expect high Tm and more transition temperatures for
pegylated protein vs non-pegylated protein?<o:p></o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'>Many Thanks,<o:p></o:p></span></font></p>
<p class=MsoNormal style='text-autospace:none'><font size=2 face="Courier New"><span
style='font-size:10.0pt;font-family:"Courier New"'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face="Courier New"><span style='font-size:10.0pt;
font-family:"Courier New"'>Rong-Rong</span></font><font size=2 color=navy
face=Arial><span style='font-size:10.0pt;font-family:Arial;color:navy'><o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 color=navy face=Arial><span style='font-size:
10.0pt;font-family:Arial;color:navy'><o:p> </o:p></span></font></p>
<div>
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face="Times New Roman"><span style='font-size:12.0pt'>
<hr size=2 width="100%" align=center tabindex=-1>
</span></font></div>
<p class=MsoNormal><b><font size=2 face=Tahoma><span style='font-size:10.0pt;
font-family:Tahoma;font-weight:bold'>From:</span></font></b><font size=2
face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma'> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <b><span style='font-weight:bold'>On
Behalf Of </span></b>John E Harlan<br>
<b><span style='font-weight:bold'>Sent:</span></b> Wednesday, May 21, 2008 2:47
PM<br>
<b><span style='font-weight:bold'>To:</span></b> John Burgner<br>
<b><span style='font-weight:bold'>Cc:</span></b> rasmb@rasmb.bbri.org;
rasmb-bounces@rasmb.bbri.org<br>
<b><span style='font-weight:bold'>Subject:</span></b> RE: [RASMB] Broken XLA
Mono</span></font><o:p></o:p></p>
</div>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
<p class=MsoNormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'><br>
</span></font><font size=2 face=Arial><span style='font-size:10.0pt;font-family:
Arial'>FYI-</span></font> <br>
<br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Last I
knew Skip was at a company called Technical Alternatives, a third party
provider of service for a variety of lab equipment.</span></font> <br>
<br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>John
Harlan</span></font> <br>
<br>
<o:p></o:p></p>
<table class=MsoNormalTable border=0 cellpadding=0 width="100%"
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<td valign=top style='padding:.75pt .75pt .75pt .75pt'>
<p class=MsoNormal><font size=3 face="Times New Roman"><span
style='font-size:12.0pt'><o:p> </o:p></span></font></p>
</td>
<td valign=top style='padding:.75pt .75pt .75pt .75pt'>
<p class=MsoNormal><b><font size=1 face=sans-serif><span style='font-size:
7.5pt;font-family:sans-serif;font-weight:bold'>"John Burgner"
<jburgner@purdue.edu></span></font></b> <br>
<font size=1 face=sans-serif><span style='font-size:7.5pt;font-family:sans-serif'>Sent
by: rasmb-bounces@rasmb.bbri.org</span></font> <o:p></o:p></p>
<p><font size=1 face=sans-serif><span style='font-size:7.5pt;font-family:
sans-serif'>05/20/2008 10:03 PM</span></font> <o:p></o:p></p>
</td>
<td valign=top style='padding:.75pt .75pt .75pt .75pt'>
<p class=MsoNormal><font size=1 face=Arial><span style='font-size:7.5pt;
font-family:Arial'> </span></font><br>
<font size=1 face=sans-serif><span style='font-size:7.5pt;font-family:sans-serif'>
To: "'John
Gunther'" <jrg@mywdo.com>, <rasmb@rasmb.bbri.org></span></font>
<br>
<font size=1 face=sans-serif><span style='font-size:7.5pt;font-family:sans-serif'>
cc: (bcc: John E
Harlan/LAKE/PPRD/ABBOTT)</span></font> <br>
<font size=1 face=sans-serif><span style='font-size:7.5pt;font-family:sans-serif'>
Subject: RE: [RASMB] Broken
XLA Mono</span></font><o:p></o:p></p>
</td>
</tr>
</table>
<p class=MsoNormal style='margin-bottom:12.0pt'><font size=3
face="Times New Roman"><span style='font-size:12.0pt'><br>
<br>
<br>
</span></font><font size=2 color="#1f497d" face=Calibri><span style='font-size:
10.0pt;font-family:Calibri;color:#1F497D'>John,</span></font> <br>
<font size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D'>I had the same thing happen with our old
XLA. I was able gently shake the monochromator while holding it in a
horizontal position and get the filter out of the light path. If
you can locate Skip Perry, who was a long-time Beckman service man, he
might be able to help out. </span></font> <br>
<font size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D'>John </span></font><br>
<font size=2 color="#1f497d" face=Calibri><span style='font-size:10.0pt;
font-family:Calibri;color:#1F497D'> </span></font> <br>
<b><font size=2 face=Tahoma><span style='font-size:10.0pt;font-family:Tahoma;
font-weight:bold'>From:</span></font></b><font size=2 face=Tahoma><span
style='font-size:10.0pt;font-family:Tahoma'> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <b><span style='font-weight:bold'>On
Behalf Of </span></b>John Gunther<b><span style='font-weight:bold'><br>
Sent:</span></b> Tuesday, May 20, 2008 7:13 PM<b><span style='font-weight:bold'><br>
To:</span></b> rasmb@rasmb.bbri.org<b><span style='font-weight:bold'><br>
Subject:</span></b> [RASMB] Broken XLA Mono</span></font> <br>
<br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Dear
AUC Techies </span></font><br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>
I picked up the Monochromator out of it's box and I heard a glass like
tinkling inside near the order filter. The order filter only moves a small
amount and won't go either way to it's normal stop. It's apparent to me that
something is broken. No one in the lab knows how this happened. The last logged
user got good results. It now will not pass any energy.</span></font> <br>
<font face=Arial><span style='font-family:Arial'> </span></font><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>The PI
called Beckman and they offered to replace it for an exchange price of
$16,000.00. </span></font><br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>
Does anyone have experience repairing/disassembling and reassembling one
of these? Any hints, tips, or suggestions? </span></font><br>
<font face=Arial><span style='font-family:Arial'> </span></font> <br>
<font size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>John
Gunther</span></font><tt><font size=2 face="Courier New"><span
style='font-size:10.0pt'>_______________________________________________</span></font></tt><font
size=2 face="Courier New"><span style='font-size:10.0pt;font-family:"Courier New"'><br>
<tt><font face="Courier New">RASMB mailing list</font></tt><br>
<tt><font face="Courier New">RASMB@rasmb.bbri.org</font></tt><br>
<tt><font face="Courier New">http://rasmb.bbri.org/mailman/listinfo/rasmb</font></tt></span></font><o:p></o:p></p>
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