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<DIV><FONT face=Arial size=2>Dear Christine, dear Colleagues,</FONT></DIV>
<DIV><FONT face=Arial size=2>it may be, that in your case
you have to work in a D<FONT
size=1>2</FONT>O - buffer, where the sed.velocity me be
reduced</FONT></DIV>
<DIV><FONT face=Arial size=2>due to higher solvent
density but the floataion may increase. Sure one have to try
different densities </FONT></DIV>
<DIV><FONT face=Arial size=2>where you may catch both
fractions in the same run or to catch one out of the
field.</FONT></DIV>
<DIV><FONT face=Arial size=2> Apro-pos the different densities may help you
</FONT></DIV>
<DIV><FONT face=Arial size=2>for the determination of the density dependent
fractions (see<SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-GB; mso-fareast-language: DE; mso-bidi-language: AR-SA">C
<STRONG>H<SPAN style="mso-spacerun: yes"> </SPAN>Huang and J Charlton<SPAN
style="mso-spacerun: yes"> </SPAN>(JBC 1971<SPAN
style="mso-spacerun: yes"> </SPAN>vol</STRONG> <STRONG>216<SPAN
style="mso-spacerun: yes"> </SPAN>8<SPAN style="mso-spacerun: yes">
</SPAN>April</STRONG> ) If you speak about purification I
understand that you intend to separate preparativly</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-GB; mso-fareast-language: DE; mso-bidi-language: AR-SA">some
fractions. At this point I like to mention that they
are Vertical rotors where the tubes have approximately the
same diameter as an UC cell.so , if you
have the run-condition , speed and
time </SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-GB; mso-fareast-language: DE; mso-bidi-language: AR-SA">of
the analytical run, you can copy this condition
for the vertical rotor. When the rotor
stands still the fractions (bands) became horizontal and
easy to suck them out.</SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-GB; mso-fareast-language: DE; mso-bidi-language: AR-SA">I
agree 100 % with Kristian Schillig about the schlieren-peak
inside the abs.phenomena, but this knowledge
will not help to separate or determe this two
different moving boundariesbut to recgnize
them. </SPAN></FONT></DIV>
<DIV><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 12pt; FONT-FAMILY: 'Times New Roman'; mso-fareast-font-family: 'Times New Roman'; mso-ansi-language: EN-GB; mso-fareast-language: DE; mso-bidi-language: AR-SA">good
luck Christine.....ariel</SPAN></FONT></DIV></BODY></HTML>