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<DIV>Attached is a figure I produced years ago for the lab. It is part of a larger study we never followed up on because we measure the density of every buffer we use in AUC. So this was one 400 mM stock solution at pH 6.9 used to make duplicate dilutions of each data point. Measurements were on an Anton Paar DMA 5000. Fits were done in Origin. I have some other temperature data as well but I have to put that in an approachable format.</DIV>
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<DIV>I encourage everyone to get a density meter and just measure each buffer you use.</DIV>
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<DIV>-------------------------------------------------------------------<BR>Dr. John J. "Jack" Correia<BR>Department of Biochemistry<BR>University of Mississippi Medical Center<BR>2500 North State Street<BR>Jackson, MS 39216<BR>(601) 984-1522 <BR>fax (601) 984-1501 <BR>email address: jcorreia@biochem.umsmed.edu <BR>homepage location: <A href="http://biochemistry.umc.edu/correia.html">http://biochemistry.umc.edu/correia.html</A><BR>dept homepage location: <A href="http://biochemistry.umc.edu/">http://biochemistry.umc.edu/</A><BR>-------------------------------------------------------------------<BR><BR><BR><BR><BR>>>> On 11/29/2007 at 8:31 AM, in message <4E1235AC63CA484CA744D086AF4B7E5C01E9BFA1@lavexb01.am.boehringer.com>, <psalois@lav.boehringer-ingelheim.com> wrote:<BR></DIV>
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<P><SPAN lang=fr-ca><FONT face=Arial size=2>Dear RASMB users</FONT></SPAN> </P>
<P><SPAN lang=fr-ca><FONT face=Arial size=2>I'm wondering if anybody has data about the viscosity/density of PIPES buffer. I looked in the archives and, if I'm correct, this topic was discussed a while ago (1998 ?)</FONT></SPAN></P>
<P><SPAN lang=fr-ca><FONT face=Arial size=2>Any info would be appreciated</FONT></SPAN> </P><BR>
<P><SPAN lang=fr-ca><FONT face=Arial size=2>Many thanks in advance</FONT></SPAN> </P>
<P><SPAN lang=fr-ca><FONT face=Arial size=2>Patrick Salois</FONT></SPAN> <BR><SPAN lang=fr-ca><FONT face=Arial size=2>Research associate</FONT></SPAN> <BR><SPAN lang=fr-ca><FONT face=Arial size=2>Boehringer Ingelheim Laval (Canada)</FONT></SPAN><SPAN lang=en-us></SPAN> </P></DIV><br>
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