<!DOCTYPE html PUBLIC "-//W3C//DTD HTML 4.01 Transitional//EN">
<html>
<head>
<meta content="text/html;charset=ISO-8859-1" http-equiv="Content-Type">
<title></title>
</head>
<body bgcolor="#ffffff" text="#000000">
<big>Hello all<br>
If you would like to measure solution densities but do not have a few K
to shell out for an Anton-Parr instrument, density/specific gravity
bottles (picnometers) are still available. You calibrate them with
mercury and the calibration lasts forever. They will give you at least
five place accuracy provided your balances do not complain. We use them
quite often but they are not meant to measure Vbar unless your proteins
are dirt cheap.<br>
best to all<br>
jack kornblatt<br>
</big><br>
<br>
<br>
<br>
John Correia wrote:<br>
<blockquote cite="mid474E8995.AECE.00D6.0@umsmed.edu" type="cite">
<meta http-equiv="Content-Type" content="text/html; ">
<meta content="MSHTML 6.00.2900.3199" name="GENERATOR">
<div>Attached is a figure I produced years ago for the lab. It is
part of a larger study we never followed up on because we measure the
density of every buffer we use in AUC. So this was one 400 mM stock
solution at pH 6.9 used to make duplicate dilutions of each data
point. Measurements were on an Anton Paar DMA 5000. Fits were done in
Origin. I have some other temperature data as well but I have to put
that in an approachable format.</div>
<div> </div>
<div>I encourage everyone to get a density meter and just measure
each buffer you use.</div>
<div> </div>
<div> </div>
<div> </div>
<div>-------------------------------------------------------------------<br>
Dr. John J. "Jack" Correia<br>
Department of Biochemistry<br>
University of Mississippi Medical Center<br>
2500 North State Street<br>
Jackson, MS 39216<br>
(601) 984-1522 <br>
fax (601) 984-1501 <br>
email address: <a class="moz-txt-link-abbreviated" href="mailto:jcorreia@biochem.umsmed.edu">jcorreia@biochem.umsmed.edu</a> <br>
homepage location: <a href="http://biochemistry.umc.edu/correia.html">http://biochemistry.umc.edu/correia.html</a><br>
dept homepage location: <a href="http://biochemistry.umc.edu/">http://biochemistry.umc.edu/</a><br>
-------------------------------------------------------------------<br>
<br>
<br>
<br>
<br>
>>> On 11/29/2007 at 8:31 AM, in message
<a class="moz-txt-link-rfc2396E" href="mailto:4E1235AC63CA484CA744D086AF4B7E5C01E9BFA1@lavexb01.am.boehringer.com"><4E1235AC63CA484CA744D086AF4B7E5C01E9BFA1@lavexb01.am.boehringer.com></a>,
<a class="moz-txt-link-rfc2396E" href="mailto:psalois@lav.boehringer-ingelheim.com"><psalois@lav.boehringer-ingelheim.com></a> wrote:<br>
</div>
<div
style="border-left: 1px solid rgb(5, 5, 5); margin: 0px 0px 0px 15px; padding-left: 7px; background-color: rgb(243, 243, 243);"><!-- Converted from text/rtf format -->
<p><span lang="fr-ca"><font face="Arial" size="2">Dear RASMB users</font></span>
</p>
<p><span lang="fr-ca"><font face="Arial" size="2">I'm wondering if
anybody has data about the viscosity/density of PIPES buffer. I looked
in the archives and, if I'm correct, this topic was discussed a while
ago (1998 ?)</font></span></p>
<p><span lang="fr-ca"><font face="Arial" size="2">Any info would be
appreciated</font></span> </p>
<br>
<p><span lang="fr-ca"><font face="Arial" size="2">Many thanks in
advance</font></span> </p>
<p><span lang="fr-ca"><font face="Arial" size="2">Patrick Salois</font></span>
<br>
<span lang="fr-ca"><font face="Arial" size="2">Research associate</font></span>
<br>
<span lang="fr-ca"><font face="Arial" size="2">Boehringer Ingelheim
Laval (Canada)</font></span><span lang="en-us"></span> </p>
</div>
<br>
<br>
<br>
<br>
Individuals who have received this information in error or are not
authorized to receive it must promptly return or dispose of the
information and notify the sender. Those individuals are hereby
notified that they are strictly prohibited from reviewing, forwarding,
printing, copying, distributing or using this information in any way.
<pre wrap="">
<hr size="4" width="90%">
_______________________________________________
RASMB mailing list
<a class="moz-txt-link-abbreviated" href="mailto:RASMB@rasmb.bbri.org">RASMB@rasmb.bbri.org</a>
<a class="moz-txt-link-freetext" href="http://rasmb.bbri.org/mailman/listinfo/rasmb">http://rasmb.bbri.org/mailman/listinfo/rasmb</a>
</pre>
</blockquote>
<br>
</body>
</html>