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<TITLE>Re: [RASMB] nucleic acid vbar</TITLE>
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<BLOCKQUOTE><FONT COLOR="#800000">Yes, the vbar of nucleic acids depends heavily on the cation(s) present, and can vary at least over the range 0.52 - 0.55 ml/g: Pearce, Rowe & Turnock, (1975) J. Mol. Biol. <B>97</B> 193-205. If the exact value matters to you, I doubt that there is any way of avoiding doing an actual measurement, using a modern DDM. <BR>
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Regards to all<BR>
<BR>
Arthur<BR>
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<BLOCKQUOTE>Bloomfield, Crothers & Tinoco show the salt dependence of vbar for Cs and NaDNA (taken from Eisenberg (1976), so .55 is a traditional starting point, not a fact.<BR>
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>>> On 8/26/2007 at 2:56 PM, in message <200708261956.l7QJuWtc008982@biochem.uthscsa.edu>, Borries Demeler <demeler@biochem.uthscsa.edu> wrote:<BR>
There seems to be a consensus to use a vbar of .55 ccm/g for DNA and RNA. Does<BR>
anyone know if there is a function that predicts variations in vbar with temperature<BR>
like there is for peptides? Or is .55 the best value to use - are there any<BR>
references on this?<BR>
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Thanks, -borries<BR>
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