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<DIV><FONT face=Arial size=2>Dear Sarah and rasmb's,</FONT></DIV>
<DIV><FONT face=Arial size=2>as dealing more than 50 years
with ultracentrifugation and SE. My first suggestion is
not to fit to any Mw</FONT></DIV>
<DIV><FONT face=Arial size=2>or masses as dublicates or
mixtures, but just to accept values you achieve direct .
Sure with more than 2 concentrations you
could define if your system is at the run
condition a nonideal or an self associaion
one,</FONT></DIV>
<DIV><FONT face=Arial size=2>or at what
concentration your dimer ?? dissociate in
monomer.Good that you made runs at
several speeds</FONT></DIV>
<DIV><FONT face=Arial size=2>that may show also at a
certain speed where it seems to have a single Mw
if this is a real one. </FONT></DIV>
<DIV><FONT face=Arial size=2>My way of analysing different
species is with the attached paper on the SEGAL program
(possible to down load)</FONT></DIV>
<DIV><FONT face=Arial size=2>There are different examples and
practical examples.</FONT></DIV>
<DIV><FONT face=Arial size=2>In case of questions Gia
and me could I hope help you....ariel</FONT></DIV>
<DIV><FONT face=Arial size=2><A
href="mailto:ariel.lustig@bluewin.ch">ariel.lustig@bluewin.ch</A></FONT></DIV>
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