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<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Hello<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>I recently analyzed some data using c(S) distribution model
on <span class=SpellE>Sedfit</span>. The distribution showed a few peaks, with
the main peak being at 4S. I floated the frictional ratio and came back with
approx 1.5. I then converted this to a <span class=GramE>c(</span>M)
distribution with the main peak now showing approx 65,000 </span></font><st1:City><st1:place><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>Daltons</span></font></st1:place></st1:City><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'>. Knowing
our monomer weight as 16,400 </span></font><st1:City><st1:place><font size=2
face=Arial><span style='font-size:10.0pt;font-family:Arial'>Daltons</span></font></st1:place></st1:City><font
size=2 face=Arial><span style='font-size:10.0pt;font-family:Arial'> I concluded
this was a tetramer.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>At the same time the same protein sample was analyzed using synchrotron
<span class=GramE>X</span> ray scattering. Their results concluded an <span
class=SpellE>octomer</span>, a two ring structure.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>With this knowledge I went back to the data and reanalyzed
using <span class=SpellE>Sedfit</span>, c(s) and <span class=GramE>c(</span>M)
again. Using the calculator function on <span class=SpellE>Sedfit</span> I calculated
that for 4S with a frictional ratio 1.5 this would be roughly the tetramer
weight, however, a frictional ratio of 2.4 for 4s gives a molecular weight
equal to the <span class=SpellE>octomer</span>. When I entered this into the
parameters of the <span class=GramE>c(</span>M) model the <span class=SpellE>rsmd</span>
values show the f/f0 of 2.4 gives a better fit, 0.07 to 0.119 for f/f0 of 1.5.<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>My question is how I could have got this answer without the knowledge
of the scattering data? Is the use of the calculator function to fit data
recommended? Especially in a sample with three peaks?<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Many thanks<o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'>Samantha <o:p></o:p></span></font></p>
<p class=MsoNormal><font size=2 face=Arial><span style='font-size:10.0pt;
font-family:Arial'><o:p> </o:p></span></font></p>
<p class=MsoAutoSig><font size=2 color=purple face=Verdana><span lang=EN-GB
style='font-size:11.0pt;font-family:Verdana;mso-fareast-font-family:Batang;
color:purple;mso-ansi-language:EN-GB;mso-no-proof:yes'>Samantha Jones<o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=2 color=purple face=Verdana><span lang=EN-GB
style='font-size:11.0pt;font-family:Verdana;mso-fareast-font-family:Batang;
color:purple;mso-ansi-language:EN-GB;mso-no-proof:yes'>MRC Prion Unit<o:p></o:p></span></font></p>
<p class=MsoAutoSig><st1:place><st1:PlaceType><font size=2 color=purple
face=Verdana><span lang=EN-GB style='font-size:11.0pt;font-family:Verdana;
mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'>Institute</span></font></st1:PlaceType><font size=2
color=purple face=Verdana><span lang=EN-GB style='font-size:11.0pt;font-family:
Verdana;mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'> of </span></font><st1:PlaceName><font size=2 color=purple
face=Verdana><span lang=EN-GB style='font-size:11.0pt;font-family:Verdana;
mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'>Neurology</span></font></st1:PlaceName></st1:place><font
size=2 color=purple face=Verdana><span lang=EN-GB style='font-size:11.0pt;
font-family:Verdana;mso-fareast-font-family:Batang;color:purple;mso-ansi-language:
EN-GB;mso-no-proof:yes'><o:p></o:p></span></font></p>
<p class=MsoAutoSig><font size=2 color=purple face=Verdana><span lang=EN-GB
style='font-size:11.0pt;font-family:Verdana;mso-fareast-font-family:Batang;
color:purple;mso-ansi-language:EN-GB;mso-no-proof:yes'>Queen Square<o:p></o:p></span></font></p>
<p class=MsoAutoSig><st1:City><st1:place><font size=2 color=purple
face=Verdana><span lang=EN-GB style='font-size:11.0pt;font-family:Verdana;
mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'>London</span></font></st1:place></st1:City><font size=2
color=purple face=Verdana><span lang=EN-GB style='font-size:11.0pt;font-family:
Verdana;mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'><o:p></o:p></span></font></p>
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style='font-size:11.0pt;font-family:Verdana;mso-fareast-font-family:Batang;
mso-bidi-font-family:Arial;color:purple;mso-no-proof:yes'>WC1N 3BG</span></font><font
size=2 color=purple face=Verdana><span style='font-size:11.0pt;font-family:
Verdana;mso-fareast-font-family:Batang;color:purple;mso-ansi-language:EN-GB;
mso-no-proof:yes'> <span lang=EN-GB><o:p></o:p></span></span></font></p>
<p class=MsoNormal><font size=3 face="Times New Roman"><span style='font-size:
12.0pt'><o:p> </o:p></span></font></p>
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