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<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Dear colleauges,<?xml:namespace prefix = o ns =
"urn:schemas-microsoft-com:office:office" /><o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Long time you heard at conferences and via
rasmb communication about the SEGAL<SPAN style="mso-spacerun: yes">
</SPAN>computer- program for sedimentation equilibrium SE evaluation. Some of
you even try to use it. <o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">The idea behind the program was to create
>> a program<SPAN style="mso-spacerun: yes"> </SPAN>for<SPAN
style="mso-spacerun: yes"> </SPAN>the<SPAN
style="mso-spacerun: yes"> </SPAN>poor <<, We mean<SPAN
style="mso-spacerun: yes"> </SPAN>for<SPAN
style="mso-spacerun: yes"> </SPAN><o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">non-specialists, who just<SPAN
style="mso-spacerun: yes"> </SPAN>want<SPAN
style="mso-spacerun: yes"> </SPAN>to<SPAN style="mso-spacerun: yes">
</SPAN>achieve<SPAN style="mso-spacerun: yes"> </SPAN>the<SPAN
style="mso-spacerun: yes"> </SPAN>molecular masses<SPAN
style="mso-spacerun: yes"> </SPAN>of the<SPAN
style="mso-spacerun: yes"> </SPAN>sample they deal with, not to worry
for<SPAN style="mso-spacerun: yes"> </SPAN>equilibrated<SPAN
style="mso-spacerun: yes"> </SPAN>or dialysed<SPAN
style="mso-spacerun: yes"> </SPAN>buffer/ blank ( not always possible) or
even<SPAN style="mso-spacerun: yes"> </SPAN>for<SPAN
style="mso-spacerun: yes"> </SPAN>conservation of mass during a run , that
is often not self evident for<SPAN style="mso-spacerun: yes">
</SPAN>biological<SPAN style="mso-spacerun: yes">
</SPAN>solutions.<o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Until now<SPAN style="mso-spacerun: yes">
</SPAN>every one could get<SPAN style="mso-spacerun: yes"> </SPAN>the<SPAN
style="mso-spacerun: yes"> </SPAN>information<SPAN
style="mso-spacerun: yes"> </SPAN>from the<SPAN
style="mso-spacerun: yes"> </SPAN>net, (see<SPAN
style="mso-spacerun: yes"> </SPAN>reference 12<SPAN
style="mso-spacerun: yes"> </SPAN>of the attached<SPAN
style="mso-spacerun: yes"> </SPAN>Segal-paper.) but<SPAN
style="mso-spacerun: yes"> </SPAN>this<SPAN
style="mso-spacerun: yes"> </SPAN>net information was uncompleted without
the theory section.<o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">WE attached<SPAN
style="mso-spacerun: yes"> </SPAN>a second<SPAN
style="mso-spacerun: yes"> </SPAN>NANOMEDICINE paper,(on a following
mail to you to prevent to overload this mail) as an
example,there are electron<SPAN style="mso-spacerun: yes">
</SPAN>micrographs of samples that<SPAN style="mso-spacerun: yes">
</SPAN>were also run<SPAN style="mso-spacerun: yes"> </SPAN>by SE and
evaluated<SPAN style="mso-spacerun: yes"> </SPAN>with<SPAN
style="mso-spacerun: yes"> </SPAN>Segal.<SPAN
style="mso-spacerun: yes"> </SPAN>This non homogene particles with
dark<SPAN style="mso-spacerun: yes"> </SPAN>background<SPAN
style="mso-spacerun: yes"> </SPAN>that represent<SPAN
style="mso-spacerun: yes"> </SPAN>low molecular<SPAN
style="mso-spacerun: yes"> </SPAN>weight<SPAN
style="mso-spacerun: yes"> </SPAN>particles and<SPAN
style="mso-spacerun: yes"> </SPAN>causes<SPAN
style="mso-spacerun: yes"> </SPAN>a huge<SPAN
style="mso-spacerun: yes"> </SPAN>base absorption in the Auc cell. Even
so, the<SPAN style="mso-spacerun: yes"> </SPAN>information<SPAN
style="mso-spacerun: yes"> </SPAN>about the<SPAN
style="mso-spacerun: yes"> </SPAN>nano- particles<SPAN
style="mso-spacerun: yes"> </SPAN>is<SPAN style="mso-spacerun: yes">
</SPAN>clear.<o:p></o:p></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">A third<SPAN style="mso-spacerun: yes">
</SPAN>FASEB paper<SPAN style="mso-spacerun: yes"> ( </SPAN>attached
separatly on a third mail), shows<SPAN style="mso-spacerun: yes">
</SPAN>how<SPAN style="mso-spacerun: yes"> </SPAN>the<SPAN
style="mso-spacerun: yes"> </SPAN>achieved<SPAN
style="mso-spacerun: yes"> </SPAN>Segal<SPAN
style="mso-spacerun: yes"> </SPAN>evaluated<SPAN
style="mso-spacerun: yes"> </SPAN>results<SPAN
style="mso-spacerun: yes"> </SPAN>open new<SPAN
style="mso-spacerun: yes"> </SPAN>horizons for<SPAN
style="mso-spacerun: yes"> </SPAN>coil-coil binding. Te past
year I presented at the London meeting the Nature
Structure LiF /Lipase paper calculated with Segal.</SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Gia and<SPAN style="mso-spacerun: yes">
</SPAN>me hope to help with this program to solve also some scientific
non-solved questions<SPAN style="mso-spacerun: yes"> </SPAN>with this
simple instrument <SPAN style="mso-spacerun: yes"> </SPAN>compared to new
soffisticated <SPAN style="mso-spacerun: yes"> </SPAN>programs.</SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Gia Machaidse gia <A
href="mailto:machaidse@unibas.ch">machaidse@unibas.ch</A></SPAN></P>
<P class=MsoNormal style="MARGIN: 0cm 0cm 0pt"><SPAN lang=EN-GB
style="mso-ansi-language: EN-GB">Ariel
Lustig <A
href="mailto:ariel.lustig@bluewin.ch">ariel.lustig@bluewin.ch</A><o:p></o:p></SPAN></P></FONT></DIV></BODY></HTML>