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Hi all-<br>
As you have heard, there is great variability in success with operating
the XL/I at 37 C. Here are a couple of generalizations from over the
years-<br>
- Oil vapor is the big problem, with the major source being the
diffusion pump<br>
- The absorbance system is far less sensitive to condensed oil vapor
than the interference system. <br>
- For the interference optics, the condensing lens (no pun intended)
tends to fog up, even if there is no overt oil deposition in the rest
of the chamber<br>
- Oil condenses preferentially at the cold spots in the chamber, so
pre-warming your rotor/cells reduces oil condensation on the samples<br>
- If you have even the slightest vacuum leak in your vacuum system, oil
deposition will be a problem<br>
Walter and David's work with the turbomolecular pump is very
encouraging!<br>
Best wishes,<br>
Tom<br>
<br>
Trushar Patel wrote:
<blockquote cite="mids464dd91.004@ccw0m1.nottingham.ac.uk" type="cite">
<pre wrap="">Dear all,
I have done experiments at 30C using XL-I and it worked superbly.
Regards,
Trushar
**********************************************
Trushar Patel
PhD Student
National Centre for Macromolecular Hydrodynamics
School of Biosciences
University of Nottingham
Sutton Bonington
LE12 5RD, UK
Tel: +44 115 9516149(Lab)
E-mail : <a class="moz-txt-link-abbreviated" href="mailto:stxtrp@nottingham.ac.uk">stxtrp@nottingham.ac.uk</a>
www: <a class="moz-txt-link-freetext" href="http://www.nottingham.ac.uk/ncmh/students/trushar.html">http://www.nottingham.ac.uk/ncmh/students/trushar.html</a>
***********************************************
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<pre wrap="">"Christine Ebel" <a class="moz-txt-link-rfc2396E" href="mailto:christine.ebel@ibs.fr"><christine.ebel@ibs.fr></a> 05/12/06 6:37 PM >>>
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<pre wrap=""><!---->Hello,
I have a bad experience with my unique run at 37°C.
I had oil condensation.
In consequence, for this run, and all the experiments after, (until the
Beckman man came and washed everything) even if I washed the filter, where
consensation was obvious, interference was unusable: it become noisy (very
noisy) after about two hours of run.
The problem is not so obvious with absorbance.
I am curious to know at what temperature we can run using interference
optics.
Christine
Christine EBEL
Institut de Biologie Structurale UMR 5075 CEA-CNRS-UJF
41 rue Jules Horowitz, 38027 Grenoble Cedex 1, France
Tel (33) (0) 4 38 78 96 38; Fax (33) (0) 4 38 78 54 94
<a class="moz-txt-link-abbreviated" href="mailto:christine.ebel@ibs.fr">christine.ebel@ibs.fr</a>
<a class="moz-txt-link-rfc2396E" href="http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm"><http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm></a>
<a class="moz-txt-link-freetext" href="http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm">http://www.ibs.fr/content/ibs_eng/presentation/lab/lbm/ebel.htm</a>
-----Message d'origine-----
De : <a class="moz-txt-link-abbreviated" href="mailto:rasmb-bounces@server1.bbri.org">rasmb-bounces@server1.bbri.org</a> [<a class="moz-txt-link-freetext" href="mailto:rasmb-bounces@server1.bbri.org">mailto:rasmb-bounces@server1.bbri.org</a>]
De la part de Dean Malencik
Envoyé : vendredi 12 mai 2006 18:28
À : <a class="moz-txt-link-abbreviated" href="mailto:RASMB@server1.bbri.org">RASMB@server1.bbri.org</a>
Objet : [RASMB] XLA run at 37 degrees
Hello,
I have been asked to do a run at 37 degrees on the XLA analytical
centrifuge. Does anyone have any pitfalls to anticipate or is it even
feasible to do a run at this temperature? Any differences if the run is a
velocity or equilibrium run?
Thanks.
Dean Malencik
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<br>
<pre class="moz-signature" cols="72">--
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University of New Hampshire
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