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<TITLE>Re: [RASMB] DMSO</TITLE>
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<BLOCKQUOTE>Hi Everyone<BR>
<BR>
Various people working in the NCMH over the last few years have done at least 100+ runs in 90% aqueous DMSO in filled Epon centerpieces without any problems at all. {reason: starch is only soluble in 90% DMSO}.<BR>
<BR>
See, for example, Richard F Tester, Trushar Patel & Stephen E Harding (2006) "Damaged starch chaaracterisation by ultracnbetrifugation" Carb Res 341 130-137 <BR>
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Arthur<BR>
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</BLOCKQUOTE>-- <BR>
*************************<BR>
Arthur Rowe<BR>
Lab at Sutton Bonington<BR>
tel: +44 115 951 6156<BR>
fax: +44 115 951 6157<BR>
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<BLOCKQUOTE><FONT COLOR="#0000FF"><FONT SIZE="2"><FONT FACE="Arial">Hi Norbert<BR>
I've done some sedimentation equilibrium experiments in charcoal filled epon cells looking at the effects of small molecule inhibitors on protein oligomerization where the drugs were dissolved in DMSO. It was a long time ago and I don't have access to my old notebooks from Merck, but I believe I went up to at least 2% DMSO. There was no effect on the centerpieces. <BR>
Jim <BR>
</FONT></FONT></FONT><HR ALIGN=CENTER SIZE="3" WIDTH="100%"><BR>
<FONT SIZE="2"><B>From:</B> rasmb-bounces@server1.bbri.org [mailto:rasmb-bounces@server1.bbri.org] <B>On Behalf Of </B>John Philo<BR>
<B>Sent:</B> Thursday, March 23, 2006 10:44 AM<BR>
<B>To:</B> rasmb@server1.bbri.org<BR>
<B>Subject:</B> RE: [RASMB] DMSO<BR>
</FONT><BR>
<FONT COLOR="#0000FF"><FONT SIZE="2"><FONT FACE="Arial">Norbert,<BR>
</FONT></FONT></FONT> <BR>
<FONT COLOR="#0000FF"><FONT SIZE="2"><FONT FACE="Arial">I'm pretty sure 0.5% DMSO is no problem with Epon, but the person who would know for certain is Natalia Markova at Biovitrum in Switzerland. She looks at small molecules that come out of drug screens and their binding to proteins and I believe she has extensively studied various issues regarding DMSO and its effects on proteins. Since she hasn't yet responded I suspect she is not on the RASMB though so I will respond to you privately with her contact information.<BR>
</FONT></FONT></FONT> <BR>
<FONT COLOR="#0000FF"><FONT SIZE="2"><FONT FACE="Arial">John<BR>
</FONT></FONT></FONT><BLOCKQUOTE><FONT SIZE="2">-----Original Message-----<BR>
<B>From:</B> rasmb-bounces@rasmb.bbri.org [mailto:rasmb-bounces@rasmb.bbri.org] <B>On Behalf Of </B>Norbert Muecke<BR>
<B>Sent:</B> Thursday, March 23, 2006 5:36 AM<BR>
<B>To:</B> rasmb@server1.bbri.org<BR>
<B>Subject:</B> [RASMB] DMSO<BR>
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</FONT><FONT FACE="Arial">Dear RASMBs,<BR>
we do have a solution with 0.5% DMSO.<BR>
Is this a problem with standard Epon chacoal-filled cells?<BR>
In the manual from Beckman it is written that DMSO interact with these cells.<BR>
</FONT><BR>
<FONT FACE="Arial">Will it be a problem in the Aluminium cells? Or with the gaskets? Or housing plugs?<BR>
We do have this problem now and a fast answer would be very helpful.<BR>
</FONT><BR>
<FONT FACE="Arial">Thank you in advance<BR>
Norbert<BR>
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