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<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff>Dear Thomas,</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff>I did sedimentation equilibrium in Toluene and n-Hexane several
years ago. Judging from the data sheet I refrained from using epon centerpieces
but rather used Kel-F mantled Aluminum centerpieces and had no problem with
swelling or distortion. These centerpieces have been in the prodiction line of
Beckman with part # 331420 many years ago.</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff>Hope this may help</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff>Claus</FONT></SPAN></DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006><FONT face=Arial
color=#0000ff></FONT></SPAN> </DIV>
<DIV dir=ltr align=left><SPAN class=718270909-21022006></SPAN> </DIV>
<DIV><FONT face=Arial color=#0000ff></FONT> </DIV>
<DIV class=Section1>
<P class=MsoAutoSig>Prof. Dr. Claus Urbanke</P>
<P class=MsoAutoSig>Medical School Hannover</P>
<P class=MsoAutoSig>Biophysical Chemistry OE 8830</P>
<P class=MsoAutoSig>D-30625 Hannover</P>
<P class=MsoAutoSig>Germany</P>
<P class=MsoAutoSig><?xml:namespace prefix = o ns =
"urn:schemas-microsoft-com:office:office" /><o:p> </o:p></P>
<P class=MsoAutoSig><o:p> </o:p></P></DIV>
<DIV><FONT face=Arial color=#0000ff></FONT> </DIV><BR>
<DIV class=OutlookMessageHeader lang=de dir=ltr align=left>
<HR tabIndex=-1>
<FONT face=Tahoma size=2><B>Von:</B> rasmb-bounces@rasmb.bbri.org
[mailto:rasmb-bounces@rasmb.bbri.org] <B>Im Auftrag von </B>Thomas Adam
Jowitt<BR><B>Gesendet:</B> Montag, 20. Februar 2006 17:25<BR><B>An:</B>
rasmb@rasmb.bbri.org<BR><B>Betreff:</B> [RASMB] Working with
toluene<BR></FONT><BR></DIV>
<DIV></DIV>
<DIV class=Section1>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial">Hello</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial"></SPAN></FONT> </P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial">I would like to
know if anyone has any experience of working with toluene in Epon centrepieces.
I think there may be an issue with swelling of the centrepiece in certain
organic solvents which I assume could cause the cells to break. As this is a
velocity study the run length is quite short, also is it possible to minimise
problems by pre-swelling the centrepiece?</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial"></SPAN></FONT> </P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial">Any advice would
be much appreciated</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial"></SPAN></FONT> </P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial">Thanks</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial"></SPAN></FONT> </P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial">Tom
</SPAN></FONT></P>
<P class=MsoNormal><FONT face=Arial size=2><SPAN lang=EN-GB
style="FONT-SIZE: 10pt; LINE-HEIGHT: 150%; FONT-FAMILY: Arial"></SPAN></FONT> </P>
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