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<DIV><SPAN class=041322417-15022006><FONT face=Arial>Dear
Jo,</FONT></SPAN></DIV>
<DIV><SPAN class=041322417-15022006><FONT face=Arial></FONT></SPAN> </DIV>
<DIV><SPAN class=041322417-15022006><FONT face=Arial>I have done experiment
using a pure protein in various concentration of glycerol (0-10%) as a project
to familiar myself with the SEDFIT software a while back. I also used
the same protein and watch how dimer dissociated in the presence of GuHCl (0-5M)
analyzed with the same software. I will share these results if anyone is
interested.</FONT></SPAN></DIV>
<DIV><SPAN class=041322417-15022006></SPAN> </DIV>
<DIV><SPAN class=041322417-15022006></SPAN><SPAN class=041322417-15022006><FONT
face=Arial>Sucequently, I was interested in how to choose scan, the # of scan
used to get the best fit and analyzed the raw data with both SEDFIT and DCDT+
and compare these results. I also did some experiments, using a dimer
protein and observe what happen in the presence of various concentration of
denaturant(GuHCl), as mentioned above, but analyzed the raw data with both
SEDFIT and DCDT+ and compare the results. I can also share these results
in case anybody is interested.</FONT></SPAN></DIV>
<DIV><SPAN class=041322417-15022006><FONT face=Arial></FONT></SPAN> </DIV>
<DIV><FONT face=Arial><SPAN class=041322417-15022006>I did these experiments as
an in house projects, Just </SPAN>t<SPAN class=041322417-15022006>o familiar
myself, as an experimentalist, not theoretician, to observe the pro and
con of using these two softwares. Enough been said.</SPAN></FONT></DIV>
<DIV><FONT face=Arial><SPAN class=041322417-15022006></SPAN></FONT> </DIV>
<DIV><FONT face=Arial><SPAN
class=041322417-15022006>Regards,</SPAN></FONT></DIV>
<DIV><FONT face=Arial><SPAN class=041322417-15022006></SPAN></FONT> </DIV>
<DIV><FONT face=Arial><SPAN class=041322417-15022006>Chris</SPAN></FONT></DIV>
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<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px" align=left><SPAN
style="FONT-SIZE: 10pt; FONT-FAMILY: Arial">--------------------------------------------------------<SPAN
class=205401418-09082005>----</SPAN></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>Christopher Chin</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>Manager,<SPAN class=205401418-09082005>
Macromolecular Assembly Core</SPAN></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>Sealy Center for Structural Biology
and <BR><SPAN lang=en-us>Molecular Biophysics</SPAN> </FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2><FONT color=#000080><FONT
color=#000000>Department of Biochemistry & Molecular
Biology</FONT></FONT></FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>5134 MRB. rt1055</FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>UTMB, Galveston, TX </FONT><A
href="mailto:cchin@utmb.edu"><FONT size=2>cchin@utmb.edu</FONT></A><FONT
size=2>, </FONT></SPAN></P>
<P class=MsoNormal style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px"><SPAN
style="FONT-SIZE: 12pt"><FONT size=2>409-772-1693, efax
630-604-3416</FONT></SPAN></P>
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style="FONT-SIZE: 12pt"></SPAN><SPAN style="FONT-SIZE: 12pt"><FONT
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